Study on biosynthesis of laminin-5 and keratinocyte attachment
| Project/Area Number |
08670956
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
MOROHASHI Masaaki Toyama Medical and Pharmaceutical University, Faculty of Medicine, Professer, 医学部, 教授 (50018719)
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUYAMA Minoru Toyama Medical and Pharmaceutical University, Instructor, 医学部, 助手 (10213787)
MATSUI Chihiro Toyama Medical and Pharmaceutical University, Lecturer, 付属病院, 講師 (10181679)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | hemidesmosome / laminin-5 / epidermolysis bullosa / 基底膜 / 細胞接着 |
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| Research Abstract |
Laminin-5 is a component of the anchoring filament in the basement membrane zone of the skin and crucial for keratinocyte attachment. Junctional epidermolysis bullosa (JEB) is an autosomal recessive skin blistering disease with both lethal and non-lethal forms, with most patients shown to have defects in laminin-5. We analyzed the location of mutations, gene expression levels, and protein chain assembly of laminin-5 heterotrimer in six JEB patients to determine how the type of genetic lesion influences the pathophsiology of JEB.Mutations within laminin-5 genes were diversely located, with the most severe forms of JEB correlating best with premature termination codons, rather than mapping to any particular protein domain. In all six JEB patients, the laminin-5 assembly intermediates we observed were as predicuted by our previous work indicating that the alpha3beta2gamma2 heterotrimer assembles intracellularly via a beta2gamma2 heterodimer intermediate. Since assembly procedes secretion, mutations that disrupt protein-protein inteructions needed for assembly are predicuted to limit the secretion of laminin-5, and likely to interfere with function. However, our data indicate that typically the most severe mutations diminish mRNA stabilty, and serve as functional null alleles that block chain assembly by resulting in either a deficiency (in the non-lethal mitis variety) or a complete abscence (in lethal Herlitz-JEB) of one of the chains needed for laminin-5 heterotrimer assembly.
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Report
(3 results)
Research Products
(3 results)
