| Project/Area Number |
08670957
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
MATSUI Chihiro Toyama Medical and Pharmaceutical University, Faculty of Medicine, Lecturer, 付属病院, 講師 (10181679)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | hemidesmosome / laminin-5 / wound healing / cyclic AMP / ヘミデスモソーム |
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| Research Abstract |
Laminin-5 is a component of the anchoring filament in the basement membrane zone of the skin. Since defects in laminin-5 result in defective anchoring filament and cause Herlitz junctional epidermolysis bullosa, laminin-5 is thought to be crucial for keratinocyte attachment. Based on our study regarding to laminin-5 assembly in cultured cell and medium, we have tried to elucidate the change of keratinocyte attachment during wound healing. We used 3mm diameter pieces of human epidermis for explant culture in medium with or without dibutyryl cyclic AMP (dbcAMP). After 48hr culture, explants were processed for section and length of outgrowth is measured on microscopy. Epidermis outgowth was accelerated at 10^<-6> and 10^<-7> M of dbcAMP.Since the number of PCNA positive nuclei in epidermis also increased at same condition, dbcAMP was seemed to stimulate keratinocyte proliferation and consequently enhance epidermisoutgrowth. This explant culture is useful in vitro svstem for assay of wound healing agents. To analyze expression of laminin-5 in basement membrane zone of explant culture, outgrowth area was too small to make cryosection. We are trying to analyze basement membrane reconstruction and keratinocyte attachment during wound healing by in vivo wound assay using hairless mouse.
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