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REPIGMENTAION OF VITILIGO AND MELANOCYTE MIGRATION -REGULATING MECHANISMS OF MELANOCYTE MIGRATION IN EPIDERMIAL-FOLLICULAR UNIT-

Research Project

Project/Area Number 08670968
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKOBE UNIVERSITY

Principal Investigator

HORIKAWA Tatsuya  KOBE UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF DERMATOLOGY,LECTURER, 医学部, 講師 (00173633)

Co-Investigator(Kenkyū-buntansha) UEDA Masato  KOBE UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF DERMATOLOGY,ASSOCIATE PROFESSOR, 医学部, 助教授 (20176598)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Keywordsmelanocyte / vitiligo / hair follicle / migration / ras / tyrosinase / PLA2 / pigment / melanocyte / outer root sheath / 毛包 / piebaldism / 色素拡大 / 紫外線 / TPA
Research Abstract

We have shown that the inactive melanocytes in the outer root sheath of the human hair follicles (ORS-MCs) are identifiable by anti-premelanosomal antibodies, but not antibodies to tyrosinase, TRP-1 and TRP-2 (J Invest Dermatol, 106 : 28-35,1996). These melanocytes could be cultivated (J derm Sci, 12 : 204,1996). We developed a new technique to cultivate human melanocytes using switching the conCentration of calcium (Pigment Cell Research, 9 : 58-62,1996). Using organ culture technique to cultivate the hairs, we found that ORS-MCs can become activated and DOPA-positive in vitro. In the hairs with activated ORS-MCs, RT-PCR revealed that tyrosinase transcript appeared after activation (Pigment Cell Res, 10 : 429,1997). Next, we found that melanocyte migration is induced by ET-1 via heterotrimeric G protein and phospholipase A2 (PLA2), and by bFGF and LTC4 via tyrosine kinase and PLA2 (J Invest Dermatol, 108 : 573,1997). Ras-transfected melanocyte study revealed that activated ras is involved in the induction of melanocyte migration (J Derm Sci, 15 : 128,1997). Activated ras-transfected melanoma cells have higher potency of migaration than parent cells, and that PLA2 is activated in ras transfected cells (J Invest Dermatol, 109 : 407,1997). The expression of E-cadherin was found to be reduced by UV irradiation (J Invest Dermatol 106 : 1320-1324,1996). We also found that melanoma cells lack either alpha or beta PKC subspecies and beta type PKC plays an important role in pholbor ester stimulation of cell growth (J Cell Physiol 167 : 406-412,1996). We believe that ORS-MCs can be activated by proper stimulants, proliferate, migrate into surrounding epidermis. Our findings will provide a useful information in establishing a new strategy of the treatment for depigmented disorders.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Horikawa T: "DOPA-negative melanocytes in the outer root sheath of human hair follicles express premelanosomal antigens but no a melanosomal antigen or the melanosome-associated glycoproteins tyrosinase,TRP-1,and TRP-2." J.Invest.Dermatol. 106. 28-35 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T: "Long term culture of melanocytes without phorbol ester:Medium switch from high calcium to low calcium following geneticin treatment effectively eliminates fibroblasts" Pigment Cell Res. 9. 58-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seline PC: "Expression of E and P-cadherin by melanoma cells decreases in progressive melanomas and following ultraviolet radiation" J.Invest.Dermatol. 106. 1320-1324 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oka M: "Deletion of specific protein kinase C subspecies in human melanoma cells" J.Cell.Physiol.167. 406-412 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T: "Pigment spread phenomenon after epidermal grafting in depigmented disorders." Proceeding of the 10th Japan-Korea Joint Meeting of Dermatology.64-66 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 西野 圭輔: "Suction blister法による表皮移植術を施行し著明な色素拡大を認めたPiebaldismの1例" 皮膚. 38. 342-346 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T,Norris DA,Johnson TW,Zekman T,Dunscomb N,Bennion SD,Jackson RL,Morelli JG: "DOPA-negative melanocytes in the outer root sheath of human hair follicles express premelanosomal antigens but not a melanosomal antigen or the melanosome-associated glycoproteins tyrosinase" TRP-1, and TRP-2.J Invest Dermatol. 106. 28-35 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T,Norris DA,Zekman T and Morelli JG: "Long term culture of melanocytes without phorbol ester : Medium switch from high calcium to low calcium following geneticin treatment effectively eliminates fibroblasts" Pigment Cell Research. 9. 58-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seline PC,Norris DA,Horikawa T,Fujita M,Middleton MH,and Morelli JG: "Expression of Eand P-cadherin by melanoma cells decreases in progressive melanomas and following ultraviolet radiation" J Invest Dermatol. 106. 1320-1324 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oka M,Ogita K,Ando H,Horikawa T,Hayashibe K,Saito N,Kikkawa U,and Ichihashi M: "Deletion of specific protein kinase C subspecies in human melanoma cells." J Cell Physiol. 167. 406-412 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishino K,Horikawa T,Ichihashi M and Mishima Y: "A case of piebaldism with pigment spread after suction blister epidermal grafting and PUVA therapy" Hihu. 38. 342-346 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T,Nishino K,Araki K,Mishima Y and Ichihashi M: "Pigment spread phenomenon after epidermal grafting in depigmented disorders" Proceeding of the 10th Japan-Korea Joint Meeting of Dermatology. 64-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T,Norris DA,Travers J,Yagi H,Ichihashi M,Morelli JG: "Endothelin-1-stimulated melanocyte migration is mediated via a pertussis toxin sensitive, phospholipase A2-dependent pathway, while leukotrien C4 and basic fibroblast growth factor mediate movement via tyrosine kinase dependent pathway." J Invest Dermatol. 108. 573 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T,Araki K,Tsuru K,Ueda M,Tsukamoto K and Ichihashi M: "Cell Migration is induced in H-ras-transfected murine melanocyte cell line Melan-A." J Dermatol Sci. 15. 128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Norris DA,Yagi H,Horikawa T,de la Houssay, Batcher E,Pfenniger KH: "Activating ras mutations in human melanoma induce migration through autonomous activation of phospholipase A2 (PLA2)" J Invest Dermatol. 109. 407 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T,Araki K,Ueda M,and Ichihashi M: "Melanocytes in the outer root sheath of plucked hair follicles become DOPA positive after cultivation" Pigment Cell Res. 10. 429 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagai H,Hayashibe Y,Wang W,Kako S,Horikawa T,Hayashi K,and Ichihashi M: "Tissue distribution of a melanoma-associated antigen D-1 immunogenic in patients with melanoma." J Derm Sci. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Horikawa T: "Pigment spread phenomenon after epidermal grafting in depigmented disorders." Proceeding of 10th Japan-Korea Joint Meeting of Deriadology. 64-66 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Horikawa T: "Endothelin-1 Stimulated melanoalte migrchm is mediated via a pertussis toxin sensitive phosphslipsse A20 dependent pathway,while leukotrien C_4 and basic fibregrowth fator mecliate movement via tyrosine Kinase clepenclent pathway," J.Invest,Dermatol.108. 573- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Horikawa T: "Migration is induced in H-ras trancfected murine melanocyte cell line Melan-A," J.Dermatol.Sci. 15. 128- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Horikawa T: "Melanocyte in the oater rost Sheath of plucked hair follicles become DOPA positive after cultivation." Pigment Cell Res.10. 429- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Norris DA: "Activating ras mutations in human melanoma induce migration through automoncks activation of phospholipase A_2 (PLA_2)." J.Invest,Dermatol.109. 407- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagai H: "Tissue distribution of a melanoma-associated autigen D-1 immunogenic in patients with melanoma" J.Dermatol.Sci.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Horikawa T: "DOPA-negative melanocytes in the outer root sheath of human hair follicles express premelanosomal antigens but not a melanosomal antigen or the melanosome-associated glycoproteins tyrosinase TRP-1 and TRP-2" J.Invest.Dermatol. 106. 28-35 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Horikawa T: "Long term culture of melanocytes without phorbol ester : Medium switch from high calcium to low calcium following genelicin treatment effectively eliminates fibrolasts" Pigment Cell Res. 9. 58-62 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seline PC: "Expression of E and P-cadherin by melanoma cells decreases in progressive melanomas and following ultraviolet radiation" J.Invest.Dermatol. 106. 1320-1324 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Oka M: "Deletion of specific protein kinase C subspecies in human melanoma cells" J.Cell.Physiol.167. 406-412 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Horikawa T: "Inactive melanocytes in the outer root sheath of the hair follicle and sebaceous gland" J.Derm.Sci.12. 204- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西野圭輔: "Suction blister法による表皮移植術を施行し著明な色素拡大を認めたPiebaldismの1例" 皮膚. 38. 342-346 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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