| Project/Area Number |
08670974
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TANAKA Yoichi Nagasaki University, School of Medicine, Lecturer, 医学部, 講師 (20231417)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENAKA Motoi Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (30281207)
YAMAMOTO Kenshi Nagasaki University, School of Medicine Hospital, Assistant, 医学部・附属病院, 助手 (90240093)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | gut / food allergy / eosinophil / IL-5 / chemokine / ovalbumin / eosonighil / in situ hybridization |
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| Research Abstract |
We got the results described below.
1. Balb/c mice were intraperitonealy immunized with ovalbumin (OVA) and alum, and high anti-OVA IgE titers were observed by passive cutaneous anapylaxis (pCA).
2. Antigen-specific significanti eosinophil infiltrations were observed in lamina propria of gut by oral challenge with OVA,and peaked at 6h.
3. The eosinophil infiltrations were significantly inhibited by preadministration with monoclonal anti-mouse IL-5 antibody.
4. IL-5 mRNA positive cells were demonstrated in lamina propria by in situ hybridization.
5. RANTES mRNA expression was observed in gut by reverse transcription polymerase chain reaction (RT-PCR), but it was not non-specific. Eotaxin mRNA was not detected by the method.
6. Transient mRNA expressions of ICAM-1 and VCAM-1 (6h after oral challenge) were observed in gut.
These results suggested important role of IL-5 in eosinophil infiltration into gut.
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