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Cellular interactions between human skin mast cell and malignant tumor of nerve origin.

Research Project

Project/Area Number 08670981
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionAkita University (1997)
Jichi Medical University (1996)

Principal Investigator

DEMITSU Toshio  Akita University, medicine, assistant professor, 医学部, 助教授 (20237027)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsMast cell / Malignant schwannoma / Cell culture / Stem cell factor / Extracellular matrices / Cellular interactions / 神経線維肉腫 / 神経ペプチド / ヒスタミン / 細胞外基質 / ピロスタグランジンD_2
Research Abstract

Human skin mast cells (HSMCs) have been implicated in a wide variety of skin disorders including urticaria, atopic dermatitis, psoriasis, and pathologic fibrosis [1]. Mast cells are also known to infiltrate around the cancer cell such as melanoma and breast cancer [2,3].
We have recently observed mast cell infiltration in malignant schwannoma (MS) arising in the patient with von Recklinghausen's disease [4]. To determine the cell to cell interactions between human skin mast cell (HSMC) and MS cell, we investigated the HSMC survival and the morphological changes when cultured with MS-derived cells isolated from the patient. Partially purified HSMCs obtained from normal adult skin and cutaneous neurofibroma by enzymatic digestion were cocultured with MS-derived cells on the coverslips. The number of HSMCs stained with crystal violet were directly counted by light microscope. HSMCs cocultured with MS-derived cell feeder layr revealed significantly increased HSMC survival compared to that with normal human skin fibroblast layr at 1 and 2 weeks. Conditioned medium of cultured MS-derived cell did not influence HSMC survival. In the morphology HSMCs cultured with MS-derived cells demonstrated spindle form in close contact with the adjacent MS-derived cells, suggesting cell to cell interactions. We failed to detect stem cell factor (SCF) mRNA in cultured MS-derived cell by RT-PCR.These results suggest that MS-derived cell is capable of supporting HSMC survival in vitro. Some factor (s) other than SCF,which is associated with direct contact between HSMCs and MS-derived cells, might relate to these observations. 1998 Elsevier Science Ireland Ltd.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] "Immunocytochemical characterization of malignant schwannoma-derived cells in culture." Journal of Dermatology. 24[1]. 1-6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Demitsu T., Murata S., Kakurai M., Kiyosawa T., Yaoita H.: "Malignant schwannoma-derived cells support human skin mast cell survival in vitro." J Dematol Sci.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 出光 俊郎: "肥満細胞の培養について ヒト皮膚肥満細胞の培養" アレルギーの臨床. 17[17]. 628-631 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Demitsu T., Murata S., Kakurai M., Kiyosawa T., Yaoita H: "Immunocytochemical characterization of malignant schwannoma-derived cells in culture." Journal of Dermatology. 24. 1-6 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Demitsu T., Murata S., Kakurai M., Kiyosawa T., Yaoita H: "Malignant schwannoma-derived cells support human skin mast cell survinal in vitro." J Dermatol Sci. (in the press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Demitsu T.: "Culture of Human Skin Mast Cell." Clinical of Allergy. 17 [7]. 628-631 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Demitsu T., Murata S., Kakurai M., Kiyosawa T., Yaoita H.: "Immunocytochemical characterization of malignant schwannoma-derived cells in culture." Journal of Dermatology. 24[1]. 1-6 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Demitsu T., Murata S., Kakurai M., Kiyosawa T., Yaoita H.: "Malignant schwannoma-derived cells support human skin mast cell survival in vitro." J Dermatol Sci.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 出光俊郎: "肥満細胞の培養について-ヒト皮膚肥満細胞の培養-." アレルギーの臨床. 17[7]. 628-631 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Demitsu T et al: "Effects of maligrant schwannoma-derived cell feeder layer on human skin mast cell culture in vitro." J.Dermatol.Sci. 10. 101- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Demitsu T et al: "Expression of extracellular matrix proteins and neuropeptides in maligrant schwannoma-derived cells in culture" J Dermatol Sci. 12. 211- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Demitsu T et al: "Mast cells in malignant schwannoma" J Invest Dermatol.105. 718- (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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