The role of stem cell factor in the development of melanocytes with regard to c-KIT and tyrosinase expression.
| Project/Area Number |
08670989
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | St.Marianna Univercity School of Medicine |
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Principal Investigator |
KAWA Yoko St.Marianna School of Medicine, Faculty of Medicine, Assistant, 医学部, 助手 (10082273)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | melanocyte / development / neural crest cell / SCF / c-KIT / ET-3 / S / mutant / DOPA / stem cell factor / neural crest / melanogenesis / mlanocyte differentiation / tyrosinase / TRP1 / TRP2 |
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| Research Abstract |
We and others previously reported that soluble stem cell factor (sSCF) and c-KIT interaction plays an important role in the development of melanocytes from neural crest cells (NCCs). It has also been shown that endothelin-3 (ET-3) promotes not only proliferation but also differentiation of NCCs. In this study, to determine the effects of ET-3 in combination with sSCF on the proliferation and differentiation of cultured NCCs into melanocytes, we cultured NCCs from wild type and 'Steel' mutant mice with ET-3, sSCF or ET-3+sSCF for 3-15 days. After immunohistochemical and DOPA staining, we counted the number of c-KIT(+) and DOPA(+) cells. In the wild type, the addition of ET-3 increased the number of c-KIT(+) and DOPA(+) cells in a dose- and time- dependent manner, while the addition of sSCF mainly increased the number of c-KIT(+) cells. Pigmented melanocytes were detected with ET-3 but not with sSCF.The combination with sSCF and ET-s showed a marked increase of c-KIT(+), DOPA(+) cells and pigmented melanocytes. In 'Steel' mutant mice, all those three cells did not appear in NCCs cultured with ET-3, although pigmented melanocytes were detected when SCF and ET-3 were both added to 'Steel' NCCs. These results suggest that there are intrinsic SCF sources in the wild type NCC explants, and that the survival of c-KIT(+) melanocyte precursers supported by SCF is essential to the acceleration of melanization by ET-3.
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Report
(3 results)
Research Products
(3 results)
