| Project/Area Number |
08671010
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | KANAZAWA UNIVERSITY |
|---|
Principal Investigator |
SHIBA Kazuhiro Kanazawa University, Radioisotope Center, Assistant Professor, アイソトープ総合センター, 助手 (40143929)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MORI Hirofumi Kanazawa University, Radioisotope Center, Professor, アイソトープ総合センター, 教授 (90019604)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | Iodovesamicol / Cholinergic neurons / Acetylcholine Transporter / Alzheimer's Disease / ベサミコール |
|---|
| Research Abstract |
The purpose of this investigation is to develop a new presynaptic cholinergic neuron mapping agent. We synthesized levorotatory isomer (-) -m-iodovesamicol ((-) mIV) radioiodinated at the meta position of the 4-phenylpiperidine moiety and evaluated the in vitro and in vivo binding affinity and specificity of [I-125] (-) -mIV for the vesamicol receptor in rat brain, furthermore, evaluated the in vivo characteristics of [I-125] (-) -mIV in the model rat of Alzheimer's disease. In vitro and In vivo binding affinity of [I-125] (-) -mIV for the vesamicol receptor (acetylcholine transporter) was very high and comparable to that of (-) -vesamicol ; the binding affinity for dopamine, serotonin, noradrenaline, acetylcholine and sigma receptors was low. These similar results were derived from saturation binding study ; Kd (18.2nM) and Bmax (660 fmol/mg of protein) of [I-125] (-) -mIV were comparable to those of (-) -vesamicol (Kd=20-30nM,Bmax=334-516fmol/mg of protein). Furthermore, double-tracer autoradiograms using [I-125] (-) -mIV and [Tc-99m] HMPAO in model rat brain of Alzheimer's disease showed a significant 11% decrease of the accumulation of [I-125] (-) -mIV in the projection cortices ipsilateral to the basalforebrain lesion in a unilateral cholinergic denervation model, which is regarded as an experimental model of Alzheimer's disease. The difference between the decrease of the accumulation of [I-125] (-) -mIV (11%) and that of [Tc-99m] HMPAO (4%) in the regions was statistically significant (P<0.01). These results suggest that radioiodinated (-) -mIV may serve as a useful radioligand for mapping presynaptic cholinergic neurons.
|
