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Fundamental Study on Assessment of Neurotransplantation Using Nuclear Medicine Imaging

Research Project

Project/Area Number 08671011
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionKANAZAWA UNIVERSITY

Principal Investigator

MORI Hirofumi  Kanazawa University, Radioisotope Center, Professor, アイソトープ総合センター, 教授 (90019604)

Co-Investigator(Kenkyū-buntansha) TSJI Siro  Kanazawa University, School of Medicine, Associate Professor, 医学部, 助教授 (70227388)
SHIBA Kazuhiro  Kanazawa University, Radioisotope Center, Assistant, アイソトープ総合センター, 助手 (40143929)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsAlzheimer's disease / Lesion of Nucleus Basalis Magnocellularis / Acetylcholine / Receptor / Neurotransplantation / Transporter
Research Abstract

Alzheimer's disease is one of most troublesome problems encountered in the elderly population. One proposed hypothesis is that a deficit in cholinergic neurotransmission in Alzheimer's disease underlies this serious symptom of the disease. Cholinergic denervation rat model by producing an unilateral lesion of nuleus basalis magnocellularis (NBM) are reported to a model of cognitive deficits, one of instructive models of Alzheimer's disease (AD). The neurotransplantation is now promising as an effective strategy for functional repair in a variety of neural systems in disorders such as Parkinson's disease. In this study autotransplantation of vagal ganglion was performed to NBM lesioned rats. The effects of cholinergic grafts on cholinergic systems evaluated by autoradiographic images in duration of 1,2 and 4 weeks after surgery. Cerebral blood flow (CBF), muscarinic acetylcholine receptor (mAChR), m1 and m2 subtype of AChR-mRNA images were obtained using 99mTc-hexamethyl-propyleneamine … More oxime (99mTc-HMPAO), [3H] quinuculidinal benzilate, 35S-labeled oligonucleotide probes by in situ hybridization respectively. Also acetylcholine transporter images were obtained using [3H] vesamicol and [3H] hemicholinium-3 (3H-HC-3). The autoradiograms were quantified by bio-imaging analyzer using imaging plate ; the regions of interesting (ROIs) were set in cortex of the affected side and unaffected side symmetrically, and the affected-side/unaffected-side ratios (AU ratio) were calculated from these mean photo-stimulated luminescence (PSL) rates. In both NBM lesioned and autoransplanted rats, there were no remarkable changes of the AU ratio at CBF,mAChR,m1mAChR-mRNA and m2mAChR-mRNA images. On the other hand, the [3H] vesamicol images of the NBM lesioned rats showed significant reduction of the mean AU ratio at 1,2 and weeks after lesioning (P<0.01). Furthermore, the mean AU ratio in autotransplanted rats gradually increased and significantly improved 4 weeks after transplantation (p<0.05). There were no remarkable changes of the AU ratio at 3H-HC-3. Clinically emission computed tomography (ECT) with radiolabeled vesamicol may be a potential procedure for the assessment of neurotransplantation therapy on patients with AD. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Shiba K: "In vitro characterization of radioiodinated (-) m-Iodovesamicol in rat cerebral membranes" Life Sciences. 59・13. 1039-1045 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuji I: "Disdrepancy between blood flow and muscarinic receptor distribution in rat brain after middle cerebral occlusion" Eur J Nuclear Medicine. 24・6. 665-669 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shiba, K.: "In vitro characterization of radioiodinated (-) m-Iodovesamicol in rat cerebral membranes." Life Sciences. 59 (13). 1039-1045 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuji, I.: "Disdrepancy between blood flow and nuscarinic receptor distribution in rat brain after middle cerebral occlusion." Eur J Nuclear Medicine. 24 (6). 665-669 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuji I: "Disdrepancy between blood flow and muscarinic receptor distribution In rat brain after middle cerebral occlusion" Eur J Nuclear Medicine. 24・6. 665-669 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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