| Project/Area Number |
08671039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | KYUSYU UNIVERSITY |
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Principal Investigator |
OTSUKA Makoto KYUSHU UNI., MEDICAL INSTITUTE OF BIOREGULATION,ASSOC.PROF., 生体防御医学研究所, 助教授 (60203840)
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| Co-Investigator(Kenkyū-buntansha) |
HATAKENAKA Masamitsu KYUSHU UNI., MED.INST.OF BIOREG., RESEARCH ASSOC., 生体防御医学研究所, 助手 (40253413)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | radiation damage / late effect / mouse kidney / cell proliferation / c-myc / C-myc |
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| Research Abstract |
We have previously described the increased proliferation and appearance of abnormally large nuclei in renal proximal tubule cells after radiation. A model has been proposed that the large nuclear cells might be dying and the increased proliferation might lead to mitotic death of these cells and cause functional damage. We measured the amount of c-myc transcript taking the ratio of densities of bands for irradiated left kidney to unirradiated right kidney after RT-PCR procedure on 24 hours after 9,12 and 15 Gy irradiation, and found positive in 1 out of 10 after 9 Gy, 6 out of 10 after 12 Gy and 9 out of 10 after 15 Gy. Measuring c-myc expression seems to be a potential very early indicator of late radiation damage of kidney.
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