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Radioimmunoimaging with recombinant light chain of human monoclonal anti-lung cancer antibody HB4C in vivo study by using lung cancer xenografted mice

Research Project

Project/Area Number 08671056
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionTokyo Women's Medical College

Principal Investigator

KUSAKABE Kiyoko  Tokyo Women's Medical College, Dpt.of Medicine Professor, 医学部, 教授 (80075473)

Co-Investigator(Kenkyū-buntansha) AIBA Motohiko  Tokyo Women's Medical College, Dpt.of Medicine Professor, 医学部, 教授 (30051775)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsRadioimmunodetection / Radioimmunotherapy / human monoclonal anti-lung cancer antibody / dHB4C5 / 放射免疫検出法 / 放射免疫治療法 / ヒトモノクローナル抗体
Research Abstract

The deglycosylated human monoclonal anti-lung cancer antibody, dHB4C5, has been establoshed it's specific recognition of lung cancer in vivo study. This dHB4C5 was labelled with In-DTPA which is adequate agent for imaging. Though higher accumulation of In-DTPA labeled dHB4C5 in the implanted tumor of mice than that's of In labeled control IgM was recognized, the remarkable uptake in liver, kidney, and spleen was also observed on images. The low affinity of In-DTPA to IgM,M900,0000 of dHB4C5, was thought to be limited application for clinical use because of high uptake in normal organs. Then, we tried to make recombinant light chain of dHB4C5, C5-L chain (C5lambda chain), and label with In-DTPA.The immunoactivity of C5lambda chain, however, was decreased compared with original C5lambda chain by using chelate DTPA.Furthermore, C5lambda chain labeled with In-111 DTPA was also accumulated in normal organs. On the otherhand, radioactive techinetium (Tc-99M), 6 hours physical half life, which is mostly used clinicaly, was applied to label with C5lambda chain. The accumulation of Tc-99m labeled C5lambda chain in tumor was shown on images 28 hours after administration clearly. Tc-99m labeled recombinant light chain C5lambda will become a promising imaging agent of lung cancer.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 日下部 きよ子: "アイソトープ療法" 臨床と研究. 74. 1704-1708 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 日下部 きよ子: "口腔領域における最新の核医学診断-基礎と臨床-" Dental Diamond. 22. 146-151 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 日下部 きよ子: "非密封RIによる内照射療法" 新医療. 3. 90-93 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 日下部 きよ子: "消化管ホルモン産生腫瘍の画像診断" 内分泌・糖尿病科. 2. 458-465 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 日下部きよ子: "アイソトープ療法" 臨床と研究. 74. 1704-1708 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 日下部きよ子: "口腔領域における最新の核医学診断-基礎と臨床-" Dental Diamond. 22. 146-151 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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