| Project/Area Number |
08671069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
TANAKA Tatsuya Asahikawa Medical College, Department of Neurosurgery, Professor, 医学部, 教授 (20108715)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Takahiro Asahikawa Medical College, Department of Neurosurgery, Assistant, 医学部, 助手 (60281896)
HASHIZUME Kiyotaka Asahikawa Medical College, Department of Neurosurgery, Assistant, 医学部, 助手 (00250580)
KUNIMOTO Masayuki Asahikawa Medical College, Department of Neurosurgery, Assistant, 医学部, 助手 (70250579)
米増 祐吉 旭川医科大学, 医学部, 教授 (30038666)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Pontine reticular formation / Kainic acid / Focal seizure / Mesencephalic reticular formation / Autoradiography / Generalized tonic seizure / Pontine reticular formation / Geueralized seizure |
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| Research Abstract |
The mode of seizure propagation was studied using generalized seizure models induced by a microinjection of kainic acid (KA) into unilateral mesencephalic reticular formation (MRF) or pontine reticular formation (PRF) of unrestrained cats or rats. Stereotaxic operation was performed in cats and a cannula for KA microinjection was implanted into MRF or PRF.One week after the surgery, KA injection was performed in freely moving condition. On EEG,a focal seizure status was initially induced at the injected site of the MRF or PRF.These seizure developed and generalized tonic seizures were induced. Clinical manifestation of MRF seizure was characterized by generalized tonic seizures followed by short clonic seizures, while that of PRF seizure was generalized tonic seizures.
In rats, a cannula was implanted stereotaxically into MRF or PRF.One week after the surgery, autoradiography was performed using 14C-deoxyglucose, 131I-iomazenil or 14C-iodoantipyrine. The mode of local glucose utilization (1CGU), distribution of benzodiazepine receptors or local cerebral blood flow (1CBF) were studied. The results demonstrated that corticofugal pathway plays an important role in the generalization of MRF seizure and medial forebrain bundle plays an important role in the generalization of the PRF seizure.
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