| Project/Area Number |
08671121
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Psychiatric science
|
|---|
| Research Institution | Hamamatsu University School of Medicine (1997)
National Center of Neurology and Psychiatry (1996) |
|---|
Principal Investigator |
IYO Masaomi Hamamatsu University School of Medicine, Associate professor, 医学部, 助教授 (50191903)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Noriyuki National Center of Neurology and Psychiatry, Chief Researcher, 心身医学研究部, 室長(研究職) (60211869)
INADA Toshiya National Center of Neurology and Psychiatry, Chief Researcher, 老人保健研究部, 室長(研究職) (00184721)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | DARPP-32 / mRNA / human |
|---|
| Research Abstract |
Central dopaminergic transmission has been hypothesized to have important roles for an etiology or pathophysiology for schizophrenia. Dopamine released in synaptic cleft binds to postsynaptic dopmaine receptors and then activates further intracellular transmission. Cyclic AMP is one of important intracellular second messengers linked to these receptors. It phosphorylates dopamine- and cyclic AMP-regulated phosphoprotein 32 (DARPP-32), a third messenger. In this study, we tried to develop the methods to detect DARPP-32 mRNA in rat brain and human blood. First, we designed a pair of primers for detecting rat DARPP-32 mRNA and successfully detected its expression in rat striatum. Second, we also designed 5 pairs of primers, referred from the reports on nucleotide sequence of rat and bovine cDNAs for DARPP-32 and amino acid sequences of human DARPP-32, for detecting human DAEPP-32 mRNA.Then, we performed PCR using these primers and human caudate cDNA library. The results of comparison between amino acid sequences of human DARPP-32 previously reported and that converted from the nucleotide sequence of our PCR products showed failure of identification of human DARPP-32 by using the primers we had designed. The present results suggest that further studies are needed to develop the methods to identify human mRNA in the blood.
|
