• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Roles of active amino acids and nitric oxides in the osmotic demyelination

Research Project

Project/Area Number 08671130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionTohoku University

Principal Investigator

OTA Kozo  Tohoku University, Dept.of Medicine, Lecturer, 医学部, 講師 (10185267)

Co-Investigator(Kenkyū-buntansha) SHOJI Masaru  Tohoku University, Dept.of Medicine, Research Associate, 医学部, 助手 (10226300)
KIMURA Tokihisa  Tohoku University, Dept.of Medicine, Asso.Professor, 医学部, 助教授 (00004945)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsOsmotic Demyelination / Glutamate / GABA / Nitric Oxide / DDAVP / SIADH / Microdialysis / Nitric Oxide / Osmotic demyelination / Organic osmolytes
Research Abstract

In order to clarify the roles of active amino acid (AA) and nitric oxides (NO) in the osmotic demyelination, which is well known to be occurred in patients with chronic severe hyponatremia and with acute correction of the hyponatremia, we mesured brain AA and NO of the SIADH rat by microdialysis (MD) method.
Materials and Methods : Sprague Dawley rats, aged 10 weeks, were used. DDAVP was continuously given for 7days using osmotic minipump to make SIADH rats. At 6 days, MD probe was inserted into rat striatum. Next day, MD probe was perfused with Ringer's solution in conscious state during hypertonic saline infusion for 60 mm through the femoral vein catheter. 7 days more after, brain was perfused and fixed for histological examination.
Results : At 2nd day, hypotonic hyponatremia was established and continued during 7 days. Hypertonic saline infusion corrected the hyponatremia within 60 min. At the hyponatrmic state, stimulatory AAs, Glutamate and Glycine, and NO were increased in the perfusates, On the other hand, inhibitory AA, GABA, was decreased. After the hypertonic saline infusion, Glutamate and Glycine, and NO were clearly decreased, but GABA was not significantly changed. In histological examination, central myelinolysis in the midbrain and pons was observed in these rats.
Discussion : It is demonstrated that chronic hyponatremia increased stimulatory AAs, Glutamate and Glycine, and neurotoxic NO in the brain. Acute correction of the hypoosmolality reduced these increase. Moreover, inhibitory AA, GABA, was decreased and not changed by osmotic correction. In conclusion, it is suggested that acute changes in the activity of Glutamate and Glycine neuron and neurotoxic NO, at least partly induced by Glutamate through the NMDA receptors, may play active roles in the development of osmotic demyelination during hyponatremic state and its correction.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Masaru Shoji, et al.: "Cortical laminar necrosis and central pontine myelinolysis in a patient with Sheehan syndrome and severe hyponatremea" Internal Medicine. 35. 427-431 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokihisa Kimura, et al.: "Impaired vasopressin suppression and enhanced atrial natriuretic hormone release following an acute water load in primary aldosteronism" European Journal of Endocrinology. 137. 154-161 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Shoji,et al.: "Genotype analysis of prepro-vasopressin signal peptide in vasopressin-producing and non-producing lung tumors" Life Sciences. 61. 2561-2566 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kozo Ota,et al.: "Effects of endothelin-induced nitric oxide on venous circulation and renal water electrolyte handling" Journal of Cardiovascular Pharmacology. 31. S128-S132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木村 時久: "Vasopessin antagonistの臨床応用" Annual Review 内分泌代謝 1996. 45-50 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 太田 耕造 他: "SIADH" 循環器科. 41.2. 155-161 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Shoji, Tokihisa Kimura, , Kozo Ota, et al.: "Cortical laminar necrosis and central pontine myelinolysis in a patient with Sheehan syndrome and severe hyponatremia" Internal Medicine. 35-5. 427-431 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokihisa Kimura, Tadasu Yamamoto.Masahiro Ohta, Kozo Ota, et al.: "impaired vasopressin suppression and enhanced atrial natriuretic hormone release following an acute water load in primary aldosteronism" European Journal of Endocrinology. 137-2. 154-161 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Shoji, Tokihisa Kimura, Kozo Ota, et al.: "Genotype analysis of prepro-vasopressin signal peptide in vasopressin-producing and -non-producing lung tumors" Life Sciences. 61-26. 2561-2666 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kozo Ota, Tokihisa Kimura, et al.: "Effects of endothelin-induced nitric oxide on venous circulation and renal water-electrolyte handling" Journal of Cardiovascular Pharmacology. 31 (Suppl.1). S128-S132 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokihisa Kimura.: "Clinical application of the vasopessin antagonists (In Japanese)" Annual Review : Endcrinology and metabolism in 1996. 45-50 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kozo Ota, Tokihisa Kimura, Masaru Shoji: "SIADH (In Japanese)" Jyunkanki-Ka. 41-2. 155-161 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokihisa Kimura, et al.: "Impaired vasopressin suppresion and enhanced atrial natriuretic hormone release following an acute water load in primary aldosteronism" European Journal of Endocrinology. 13. 154-161 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kozo Ota, et al.: "Effects of endothelin-induced nitric oxide on venous circulation and renal waterelectrolyte handing" Journal of Cardiovascular Pharmacology. 31・Suppl.1. S128-S132 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 太田耕造 他: "SIADH" 循環器科. 41・2. 155-161 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木村時久 他: "SIADHの薬物療法" ホルモンと臨床. 45・4. 341-347 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Shoji, et al.: "Cortical laminar necrosis and central pontine myelinolysis in a Patient with sheehan syndrome and severe hyponatremia" Internal Medicine. 35(5). 427-431 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tokihisa Kimura, et al.: "Impaired vasopressin suppression and enhanced atrial natriuretic hormone release following an acute water load in primary aldosteronism" European Journal of Endocrinology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahiro Ohta, et al.: "Effects of osmotic challenge on amino acids release in the hypothalamus, plasma vasopressin release and cardiovascular function in conscious ra" Brain Research. (in press).

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi