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Analysis of mechanism of goiter formation ; involvement of apoptosis

Research Project

Project/Area Number 08671173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionNagasaki University

Principal Investigator

YOKOYAMA Naokata  Nagasaki University, School of Medicine, Hospital, Assistant, 医学部・附属病院, 助手 (10240219)

Co-Investigator(Kenkyū-buntansha) KOJI Takehiko  Nagasaki University, School of Medicine, Associate Professor, 医学部, 助教授 (30170179)
KIRIYAMA Takeshi  Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (10264219)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGoiter / Apoptosis / Fas / Fas ligand / Cell proliferation / ヨード
Research Abstract

Apoptosis, a physiological process of cell death, may modulate the mass of the thyroid gland. We investigated the role of apoptosis and the possible involvement of Fas/Fas ligand (FasL) system in apoptosis during goiter formation and involution in a rat model of goiter. Rats were fed a low iodine diet (LID) and a goitrogen, 6-propy1-2-thiouracil (PTU), to induce goiter. Rats with goiter were then fed a high iodine diet (HID) to study the phase of involution. We examined the presence of apoptosis by electron microscopy (EM) and terminal deoxy-UTP nick end labeling (TUNEL). We also investigated the association between Fas and FasL expression and thyrocyte apoptosis using immunohistochemistry and western blotting. To evaluate the proliferation of thyrocytes, proliferating cell nuclear antigen (PCNA) was examined immunohistochemica11y. The number of apoptotic cells increased during goiter formation and the early stage of involution, which were also associated with increased number of Fas-positive thyrocytes and some of these cells contained TUNEL-positive nuclei. However, the expression of FasL was almost constant throughout the experiment. PCNA/TUNEL ratio markedly increased during goiter formation but decreased particularly during the late stage of goiter involution. Our results indicate that apoptosis of thyrocytes is a main factor of cell loss during goiter formation and involution, and suggest that the Fas/fasL system is involved in the induction of apoptosis of these cells. Moreover, the delicate balance between apoptosis and cell proliferation may play an important role in the control of thyroid gland mass.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Y.Shirahige: "Immunological detection of nm23-Hl/NDP kinase in childhood thyroid carcinoma." Oncology Rep. 4. 285-288 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Nishihara: "A case of adrenal insufficiency due to acquired hypothalamic CRH deficiency." Endocrine J. 44(1). 121-126 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Ejima: "Cultured oncogenic osteomalacia tumor cells produce a factor(s) that inhibits osteocalcin production by osteoblastic cells." Oncology Rep. 4. 1327-1330 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Ashizawa: "Prevalence of goiter and urinary iodine excretion levels in children around Chernobyl." J Clin Endocrinol Metab. 82(10). 3430-3433 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Kimura: "Thyroid dysfunction in patients with amyloid goitre." Clin Endocrinol. 46. 769-774 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Tominaga: "International differences in approaches to I-131 therapy for Graves' disease:case selection and restrictions recommended to patients in Japan,Korea,and China." Thyroid. 7(2). 217-220 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagataki: "Werner and Ingbar's THE THYROID" Lippincott-Raven, 1,124 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagataki: "Pharmacotherapeutics of the Thyroid Grand" Springer, 370 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Shirahige, J.Irie, K.Ashizawa, N.Yokoyama, N.Ishikawa, T.Mimura, K.Ito, S.Nagataki: "Immunohistochemical detection of nm23-H1/NDP kinase in childhood thyroid carcinoma." Oncology Reports. 4. 285-288 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Nishihara, H.Kimura, T.Ishimaru, T.Kiriyama, N.Yokoyama, S.Yamashita, S.Nagataki: "A case of adrenal insufficiency due to acquired hypothalamic CRH deficiency" Endocr J. 44. 121-126 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Ejima, T.Kiriyama, K.Fujiyama, Y.Abe, K.Ashizaw, T.Tominaga, N.Sera, M.Tamura, N.Yokoyama, S.Nagataki: "Cultured oncogenic osteomalacia tumor cells produce a factor(s) that inhibits osteocalcin production by osteoblastic cells" Oncology Reports. 4. 1327-1330 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Ashizawa, Y.Shibata, S.Yamashita, H.Namba, M.Hoshi, N.Yokoyama, M.Izumi, S.Nagataki: "Prevalence of goiter and urinary iodine excretion levels in children around Chernobyl" J Clin Endocrinol Metab. 82. 3430-3433 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Kimura, S.Yamashita, K.Ashizawa, N.Yokoyama, S.Nagataki: "Thyroid dysfunction in patients with amyloid goitre" Clinical Endocrinol. 46. 769-774 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Tominaga, N.Yokoyama, S.Nagataki, B.Y.Cho, C.S .Koh, J.L.Chen, Y.Shi: "International differences in approaches to ^<31>I therapy for Graves' disease : case selection and restrictions recommended to patients in Japan, Korea, and China." Thyroid. 7. 217-220 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagataki, N.Yokoyama: Autoregulation ; Effects of iodide. L.E.Braverman, R.D.Utiger, eds. THE THYROID (7th edition). Philadelphia-New York, Lippincott-Raven., 241-247 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nagataki, N.Yokoyama: Iodine ; Metabolism and pharmacology, A.P.Weetman, A.Grossman, eds.Pharmacotherapeutics of the Thyroid Gland.Berlin, Springer, 171-188 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Shirahige: "Immunological detection of nm23-H1/NDP kinase in childhood thyroid carcinoma." Oncology Rep. 4. 285-288 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Nishihara: "A case of adrenal insufficiency due to acquired hypothalamic CRH deficiency." Endocrine J. 44(1). 121-126 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Ejima: "Cultured oncogenic osteomalacia tumor cells produce a factor (s) that inhibits osteocalcin production by osteoblastic cells." Oncology Rep. 4. 1327-1330 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Ashizawa: "Prevalence of goiter and urinary iodine excretion levels in children around Chernobyl." J Clin Endocrinol Metab. 82(10). 3430-3433 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Kimura: "Thyroid dysfunction in patients with amyloid goitre." Clin Endocrinol. 46. 769-774 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Tominaga: "International differences in approaches to I-131 therapy for Graves' disease : case selection and restrictions recommended to patients in Japan, Korea, and China." Thyroid. 7(2). 217-220 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Nagataki: "Pharmacotherapeutics of the Thyroid Grand" Springer, 370 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Tominaga: "International differences in approaches to I-131 therapy for Graves'." Thyroid. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Shirahige: "Immunological detection of nm23-H1/NDP kinase in childhood thyroid carcinoma." Oncology Report. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Nagataki: "The Thyroid(7th edition)" Lippincott-Raven, 7 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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