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The Superoxide-generating System and its Function in Human B Lymphocytes

Research Project

Project/Area Number 08671209
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionThe University of Tokyo

Principal Investigator

KOBAYASHI Sonoko  Inst.of Med.Sci., Research Associate, 医科学研究所, 教務職員 (00013764)

Co-Investigator(Kenkyū-buntansha) KANEGASAKI Shiro  Inst.of Med.Sci.Professor, 医科学研究所, 教授 (10012767)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsB lymphocytes / CGD / surface immunoglobulin / Ig class switching / EBV-BCL / superoxide-generating system / superoxide / CGD(慢性肉芽腫症) / surface Lg / Lg class switch
Research Abstract

We have shown that human peripheral B lymphocytes contain cytochrome b558 (gp91-, and p22-phox) and cytosolic proteins, p47-and p67-phox, all are known to be essential for the superoxide generation. The amounts of these components in B lymphocytes were much lower than than those in neutrophils, which may reflect the weak superoxide-generating activity of B lymphocytes. Mean amounts (n=8) of gp91-, p22-, p47-and p67-phox in peripheral B lymphocytes as calculated from intensity of the blots were 1.1%, 2.6%, 17.9%and3.9%, relative to those in neutrophils on the basis of cell number. Epstein-Barr virus (EBV) -transformed cell lines derived from normal B lymphocytes also possessed cytochrome b558 and two cytosolic proteins.
Based on our observation that EBV-tranaformed B cell lines derived from CGD patients did not show the activity, in contrast to those obtained from normal individuals we concluded that generation of the superoxide anion by B cell lines was due to the presence of the same system as that in phagocytes. Based on our observation that EBV-transformed B cell lines derived from CGD patients did not show the activity, in contrast to those obtained from normal individuals.
Since cross-linking of surface immunoglobulins activates the superoxide-generating system in B lymphocytes, it is expected that the superoxide anion is released when the cells are exposed to a specific antigen or when the cells interact antigen-dependently with T lymphocytes. In the latter case, cross-linking of HLA-DR on the B lymphocytes may also take place. It is possible, therefore, that generated superoxide anion modulates immune responses either by affecting antigen-processing or by promoting growth of T lymphocytes. Further studies are needed to elucidate its role.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Park, M・Y., S, Imajoh-Ohmi, H, Nunoi & S, Kanegasaki.: "Synthetic peptides corresponding to various hydrophilic regions of the l-erge subunit of cytochroine b558 inhibitsuperoxide generation in a cell free sy-stem from neutrophils." Biochem.Biophys.Res.Commun.234. 531-536 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Faizumessa, N.N., T.Tsuchiya, S.Kan-egasaki, et al.: "A 25kb deletion in the S region of the cytochrome b558 heavy chain gene (cY13B)in a patient with X-linked chron-ic granulometous disease" Hum Genet. 99. 469-473 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Park, M-Y., lmajoh-Ohmi S., Nunoi H.and Kanegasaki S.: "Synthetic peptides corresponding to various hydrophilic regions of the large subunit of cytochrome b558 inhibit superoxide generation in a cell free system from neutrophils." Biochem. Biophys. Res. Commun.234. 531-536 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Faizunnessa, N.N., Tsuchiya T., Kumatori k., Kurozumi H., Imajoh-Ohmi S., Kanegasaki S.and Nakamura M.: "A 25-kb deletion in the 5'region of the cytochrome b558 heavy chain gene (CYBB) in a patient with X-linked chronic granulomatous disease." Hum Genet. 99. 469-473 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Park,M・Y., S.Imajoh-Ohmi H.Nunai & S.Kanegasaki: "Synthetic paptides corresponding to various hydnophilic regions of the large subunit of cytochrome b_<558> inhibit supcnoxide generation in a cell free system fhom neutvoplils." Biochem.Bioplujs,Res.Commun.234. 531-536 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Faizunnesa N.N., T.Tsuchiya, S.Kanegasaki et al.: "A 25-kb delation in the 5'region of the cytochnome 6558 heavy chain jene (CYBB)in a patient with X-1inked chnonic greanulomatous disease" Hum Genet. 99. 469-473 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kanegasaki,S.: "Superoxide generating system in plagocytes and B lymphocytes" Bio Factors 5. 5. in press (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yu,D.,S.Imajoh-Ohmi,K.Akagawa & S.Kanegasaki: "Suppression of superoxide-generating ability during differentiation of monocytes to dendritic cells" J.Biochm.119. 23-28 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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