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Analysis of FAP-1 gene undegoing prevention of apoptosis

Research Project

Project/Area Number 08671250
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionJichi Medical School

Principal Investigator

HATAKE Kiyohiko  JICHI MEDICAL SCHOOL,DEPARTMENT OF INTERNAL MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (80192699)

Co-Investigator(Kenkyū-buntansha) TOMIZUKA Hiroshi  JICHI MEDICAL SCHOOL,DEPARTMENT OF INTERNAL MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (00285785)
TERUI Yasuhito  JICHI MEDICAL SCHOOL,DEPARTMENT OF INTERNAL MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (10285786)
OHTSUKI Tetuya  JICHI MEDICAL SCHOOL,DEPARTMENT OF INTERNAL MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (60275691)
星野 充明  自治医科大学, 医学部, 助手 (70275692)
角田 純一  自治医科大学, 医学部, 助手 (20245054)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsApoptosis / endothelial cell derived interleukin-8 / T cell leukemia / FAP-1 / Fas / シグナル伝達 / HIV
Research Abstract

FAP-t(Fas-Associated Protein-1) is one of the apoptotic genes which involves cell death of T cell, and acts with Fas antigen. We analized and sorted four subfractions of hematopoietic stem cells f rom human bone marrow cells according to the expression of CD34 and other differentiation markers. FAP-1 has been expressed in both CFU-GM and BFU-E.We demonstrated that most immature fraction, such as 0D34+CD33- cells, showed the differences among the genes of bcl-2 families. As FAP-1 is related to T cell apoptosis, we investigated the mechanism of apoptosis in T cell leukemia. Three proteins were purified from leukemia cell-conditioned media which was cultured with PDBu. One of them was identified as end othelial cell-derived interleukin-8 (IL-8E) from N-terminal analysis of purified protein.
When we observed an interaction bewteen endothelial cells and T cell leukemia cells, IL-8 was released and its apoptosis was induced. We are now investigating the mechanism. It is important to investigate whether decoy receptor for Fas ligand is involved in activated T cell apoptosis rather than FAP-1.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (66 results)

All Other

All Publications (66 results)

  • [Publications] Shimura M, Hatake K, et al.: "Characterization of room temperature induced apoptosis in HL-60." FEBS Lett.417. 379-384 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamada M, Hatake K, et al.: "Properties of murine stroma induced by M-CSF." J.Cell Physiol. 173. 1-9 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohno R, Hatake K, et al.: "Human urinary macrophage colony-stimulating factor reduces the incidence and duration of febrile neutropenia and shortens the period required to finish three courses of intensive consolidation chemotherapy in acute myeloif leukemia." J Clin Oncol. 15. 2954-2965 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Muroi K, Hatake K.et al.: "No CD117 expression in B progenitors in the regenerating bone marrow." Int J Hematol. 66. 245-247 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohya K, Hatake K, et al: "SOCS-1/JAB/SSl-1 can bind to and suppress Tec protein-tyrosine kinase." J Biol Chem. 272. 27178-27182 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatake K, Tomizuka H, et al.: "Rare but important adverse effects of all-trans retinoic acid in acute promyelocytic leukemia and their management." J Hematol. 66. 13-19 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohtsuki T, Hatake K, et al.: "Expression of Src-like adapter protein mRNA is induced by all-trans retinoic acid." Biochem Biophys Res Comm.230. 81-84 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakamura M, Hatake K, et al.: "Single glycosyltransferase, core 2 b1-6-N-acetylglucosamiyltransferase, regulates cell surface sialy-Lex expression level in humna pre-B lymphocytic leukemia cell line KM3 treated with phorbolester" J Biol Chem. 273. 26779-26789 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzu S, Hatake K, et al.: "Identification of alternatively spliced transcripts encoding murine macrophage colony-stimulating factor." Biochem Biophys Res Comm. 245. 120-126 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamashita Y, Hatake K, et al.: "Tec and JAK2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription." Blood. 91. 1496-1507 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terui Y, Hatake K.: "Activated endothelial cells induces apoptosis in leukemic cells by endothelial interleukin-8." Blood. 92. 2672-2680 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terui Y, Hatake K.: "Identification of a novel apoptosis-inducing factor derived from leukemic cells : Endothelial interleukin-8, but not monocyte-derived, induces apoptosis in leukemic cells." Biochem Biophy Res Comm. 243. 407-411 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terui Y, Hatake K, et al.: "Bcl-X ls a Regulatory Factor of Apoptosis and Differentiation in Megakaryocytic Lineage Cells." Exp Hematol. 26. 236-244 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomizuka H, Hatake K, et al.: "CD95 predicts responsiveness to tretinoin in acute promyelocytic leukemia." Int J Mol Med. 1. 207-211 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikeda T, Hatake K, et al.: "IL-13 as well as IL-4 induces monocytes/macrophages and a monoblastic cell line(UG3)to differentiate into multinucleated giant cells in the presence of M-CSF." Biochem Biophys Res Comm. (in press.). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Igarashi H, Hatake K, Tomizuka H, et al.: "High serum level of macrophage colony-stumlating factor and granulocytic colony-stimulating factor in Kawasaki disease." Brit J Haematol. (in press.). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka-Douzono M, Hatake K, et al.: "The in vivo stimulatory effect of macrophage colony-stimulating factor on the number of stroma-initiating cells." J Cell Physiol. (in press.). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimura M, Hatake K, et al.: "Micronuclei formation and aneuploidy induced by Vpr, an accessory gene of human immunodeficiency virus type 1." FASEB J. (in press.). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimura M,Hatake K,et al.: "Characterization of room temperature induced apoptosis in HL-60." FEBS Lett.417. 379-384 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamada M,Hatake K,et al.: "Properties of murine stroma induced byM-CSF." J.Cell Physiol.173. 1-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohno R,Hatake K,et al.: "Human urinary macrophage colony-stimulating factor reduces the incidence and duration of febrile neutropenia and shortens the period required to finish three courses of intensive consolidation chemotherapy in acute myeloif leukemia." J Clin Oncol. 15. 2954-2965 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Muroi K,Hatake K,et al.: "No CD117 expression in B progenitors in the regenerating bone marrow." Int J Hematol.66. 245-247 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohya K,Hatake K,et al.: "SOCS-1/JAB/SSI-1 can bind to and suppress Tec protein-tyrosine kinase." J Biol Chem.272. 27178-27182 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatake K,Ohtsuki T,Tomizuka H,et al.: "Rare but important adverse effects of all-trans retinoic acid in acute promyelocytic leukemia and their management." Int J Hematol.66. 13-19 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohtsuki T,Hatake K,Tomizuka H,Terui Y,et al.: "Expression of Src-like adapter protein mRNA is induced by all-trans retinoic acid." Biochem Biophys Res Comm.230. 81-84 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terui Y,Hatake K,et al.: "Activated endothelial cells induces apoptosis in leukemic cells by endothelial interleukin-8." Blood. 92. 2672-2680 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Muroi K,Hatake K,et al.: "CD56 expression in B cell neoplasm." Leukemia Res. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzu S,Hatake K,et al.: "Identification of alternatively spliced transcripts encoding murine macrophage colony-stimulating factor." Biochem Biophys Res Comm.245. 120-126 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terui Y,Tomizuka H,Ohtsuki T,Hatake K.: "Identification of a novel apoptosis-inducing factor derived from leukemic cells : Endothelial interleukin-8, but not monocyte-derived, induces apoptosis in leukemic sells." Biochem Biophy Res Comm.243. 407-411 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terui Y,Hatake K,et al.: "Bcl-X Is a Regulatory Factor of Apoptosis and Differentiation in Megakaryocytic Lineage Cells." Exp Hematol.26. 236-244 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatake K,Tomizuka H,Terui Y,et al.: "Apoptosis-gene expression in hematopoietic system : Normal and pathological conditions." Int J Mol Med.1. 121-129 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomizuka H,Hatake K,et al.: "CD95 predicts responsiveness to tretinoin in acute promyelocytic leukemia." Int J Mol Med.1. 207-211 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kasahara T,Oda T,Hatake K,et al.: "IL-8 and MCP-1 production by a human glioblastoma cell line, T98G in coculture with monocytes : Requirement of mococytes-associated IL-1alpha." Eur Cytokine Net.9. 47-55 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamashita Y,Hatake K,et al.: "Tec and JAK2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription." Blood. 91. 1496-1507 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakamura M,Hatake K,et al.: "Single glycosyltransferase, core 2 b1-6-N-acetylglucosamiyltransferase, regulates cell surface sialyl-Lex expression level in human pre-B lymphocytic leukemia cell line KM3 treated with phorbolester." J Biol Chem.273. 26779-26789 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka-Douzono M,Hatake K,et al.: "The in vivo stimulatory effect of macrophage colony-stimulating factor on the number of stroma-initiating cells." J Cell Physiol. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Igarashi, Hatake K,et al.: "High serum level of macrophage colony-stumlatingfactor and granulocytic colony-stimulating factor in Kawasaki disease." Brit J Haematol. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimura M,Hatake K,et al.: "Micronuclei formation and aneuploidy induced by Vpr, an accessory gene of human immunodeficiency virus type 1." FASEB.J. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikeda T,Hatake K,et al.: "IL-13 as well as IL-4 induces monocytes/macrophages and a monoblastic cell line (UG3) to differentiate into multinucleated giant cells in the presence of M-CSF." Biochem Biophys Res Comm.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakamura M,Hatake K,et al.: "Single glycosyltransferase,core 2 b1-6-N-acetylglucosamiyltransferase,regulates cell surface sialyl-Lex expression level in human pre-B lymphocytic leukemia cell line KM3 treated with phorbolester." J Biol Chem. 273. 26779-26789 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzu S,Hatake K,et al.: "Identification of alternatively spliced transcripts encoding murine macrophage colony-stimulating factor." Biochem Biophys Res Comm. 245. 120-126 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamashita Y,Hatake K,et al: "Tec and JAK2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription." Blood. 91. 1496-1507 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Terui Y,Hatake K.: "Activated endothelial cells induces apoptosis in leukemic cells by endothelial interleukin-8." Blood. 92. 2672-2680 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Terui Y,Hatake K.: "Identification of a novel apoptosis-inducing factor derived from leukemic cells: Endothelial interleukin-8,but not monocyte-derived,induces apoptosis in leukemic cells." Biochem Biophy Res Comm. 243. 407-411 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Terui Y,Hatake K,et al.: "Bcl-X is a Regulatory Factor of Apoptosis and Differentiation in Megakaryocytic Lineage Cells." Exp Hematol. 26. 236-244 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomizuka H,Hatake K,et al.: "CD95 predicts responsiveness to tretinoin in acute promyelocytic leukemia." Int J Mol Med. 1. 207-211 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ikeda T,Hatake K,et al.: "IL-13 as well as IL-4 induces monocytes/macrophages and a monoblastic cell line(UG3) to differentiate into multinucleated giant cells in the presence of M-CSF." Biochem Biophys Res Comm. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Igarashi H,Hatake K,Tomizuka H,et al.: "High serum level of macrophage colony-stumlating factor and granulocytic colony-stimulating factor in Kawasaki disease." Brit J Haematol. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka-Douzono M,Hatake K,et al.: "The in vivo stimulatory effet of macrophage colony-stimulating factor on th number of stroma-initiating cells." J Cell Physiol. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shimura M,Hatake K,et al.: "Micronuclei formation and aneupioldy induced by Vpr,an accessory gene of human immunodeficiency virus type 1." FASEB J. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Terui Y.,Hatake K., et al.: "Identification of a novel apoptosis-inducing factor derived from leukemic cells:Endothelial interleukin-8,but not monocyte-derived,induces apoptosis in leukemic cells." Biochem.Biophy.Res.Comm.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Terui Y.,Hatake K., et al.: "Bcl-X Is a Regulatory Factor of Apoptosis and Differentiation Megakaryocytic Lineage Cells." Exp.Hematol.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hatake K., et al.: "Apoptosis-gene expression in hematopoietic system:Normal and pathological conditions." Int.J.Mol.Med.1. 121-129 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomizuka H.,Hatake K., et al.: "CD95 predicts responsiveness to tretinoin in acute promyelocytic leukemia." Int.J.Mol.Med.1. 207-211 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kasahara T.,Hatake K., et al.: "IL-8 and MCP-1 production by a human glioblastoma cell line,T98G in coculture with monocytes;Requirement of mococytes-associated IL-la." Eur.Cytokine.Net.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimura M.,Hatake K., et al.: "Characterization of room temperature induced apoptosis in HL-60." FEBS Lett.417. 379-384 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamada M.,Hatake K, et al.: "Properties of murine stroma induced by M-CSF." J.Cell Physiol.173. 1-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamashita Y.,Hatake K., et al.: "Tec and JAK2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription." Blood. (in press.). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohya K.,Hatake K., et al.: "SOCS-1/JAB/SSI-1 can bind to and suppress Tec protein-tyrosine kinase." J.Biol.Chem.272. 27178-27182 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohtsuki T.,Hatake K., et al.: "Expression of Src-like adapter protein mRNA is induced by all-trans retinoic acid." Biochem.Biophys.Res.Comm.230. 81-84 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hatake,K.,et al.: "Rare but important adverse effect of all-trans retinoic acid in acute promyelocytic leukemia." Int.J.Hematol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohtsuki,T.,Hatake,K.,Ikeda,M.,Temizuka,H.,Torui,Y.,Uwai,M.,Miura,Y: "Expression of Src-like adapter protein mRNA is induced by all-trans retinoic acid." Biochem.Biophys.Res.Comm.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miyazato,A.,Yamashita,Y.,Hatake,K.,Miura,Y.,Ozawa,K.,Mano,H.: "Tec protein tyrosine kinase is involved in the signaling mec hanism of granulocyte colony-stimulating factor." Cell Growth & Differentiation.7. 1135-1139 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Izuml,T.,Ohtsuki,T.,Ohya,K-I.,Ogawa,Y.,Yoshida,M.,Muroi,K.,Imagawa,S.,Hatake,K.,Kuriki,K.,Saito,K.,Miura,Y.: "Therapy-related leukemia with a novel 21q22 rearrangement." Cancer Genet Cytogenet.90. 45-48 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hatake,K.,Ohtsuki,T.,et al.: "Tretinoin induces bone marrow collagenous fibrosis in acute promyeiocytic leukaemia : New adverse effect but reversible." Brit.J.Haematol.93. 646-649 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka,M.,Suda,I.,Haze,K.,Nakamura,N.,Sato,K.,Kimura,F.,Motoyoshi,K.,Mizuki,M.,Tagawa,S.,et al.: "Fas ligand in human serum." Nature Medicine.2. 317-322 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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