| Project/Area Number |
08671266
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SUZUKI Hidenori The Tokyo Metropolitan Institute of Medical Science, Department of Cardiovascular Research, Researcher, 循環器病研究部門, 研究員 (30158977)
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| Co-Investigator(Kenkyū-buntansha) |
TANOUE Kenjiro The Tokyo Metropolitan Institute of Medical Science, Department of Cardiovascula, 循環器病研究部門, 研究員 (30014137)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | platelets / adhesion / collagen / immunoelectron microscopy / alphallbbeta3 integrin / fibrinogen / FAK / focal adhesion / フィブリノゲン |
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| Research Abstract |
Initial contact of platelets with collagen is mediated via alpha2beta1 integrin.alphallbbeta3 is involved in platelet spreading. However, the precise mechanism leading to platelet spreading and firm adhesion on collagen are less understood. We have found by immuno-scanning electron microscopy that the platelets adherent to immobilized collagen secreted fibrinogen which then settled onto the cover soip and worked as an anchorage/ligand for alphallbbeta3 during platelet spreading.
Next, we have examined the cytoskeletal patterns in human platelets adherent to collagen using immunofluorescence microscopy. Vinculin was detected as aligned patches, and located along actin filaments. Although talin was not detected at the ends of actin filaments, it was concentrated at the periphey of the platelets where the platelets were in close contact with adjacent platelets. pp125^<FAK> was detected as small spots, all of which, as a whole, formed a ring at the central areas of adherent platelets. These results suggest that the adherent platelets fail to form typical focal adhesion.
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