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The roles of vascular permeability factor/vascular endothelial growth factor in mesangial proliferative glomerulonephritis

Research Project

Project/Area Number 08671286
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionKobe University

Principal Investigator

IIJIMA Kazumoto  Kobe University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (00240854)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Norishige  Kobe University, Faculty of Medicine, Professor, 医学部, 教授 (10158412)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsVascular permeability factor / Vascular endothelial growth factor / Renal biopsy specimen / Mesangial cells / alpha-smooth muscle actin / Mesangial proliferative glomerulonephritis / α-Smooth muscle achin / 腎生検組織 / α-smooth muscle actin
Research Abstract

Vascular permeability factor (VPF), also known as vascular endothelial growth factor (VEGF), is a multifunctional cytokine involved in angiogenesis, inflammation and wound healing. VPF/VEGF has been reported to be produced only by glomerular podocytes in glomeruli and we found that it si produced by human cultured mesangial cells (MC). Therefore, we performed immunohistochemical analysis, using indirect immunofluorescence and in situ hybridization, of VPF/VEGF in normal kidneys (n=7) and biopsy specimens taken from 83 patients with renal diseases, including mesangial proliferative glomerulonephritis (PGN,n=58), to examine whether VPF/VEGF is produced by MC in human PGN.In all the normal subjects and all the patients except those with PGN (disease controls), VPF/VEGF protein and mRNA were detected mainly in podocytes, indicating that VPF/VEGF was produced mainly by podocytes. However, in some PGN patients, VPF/VEGF protein was demonstrated clearly in MC as well as in podocytes, as some … More of the VPF/VEGF was co-localized with alpha-smooth muscle actin, a marker of activated MC,and VPF/VEGF mRNA was expressed by MC and podocytes. Mesangial VPF/VEGF expression in PGN patients with early lesions (predominant mesangial hypercellularity) was enhanced significantly compared with that in those with later lesions (similar degree of mesangial hypercellularity and increased matrix, p<0.01, or predominant mesangial matrix increase, p<0.05), that in disease controls and normal subjects (both p<0.01). The time between biopsy and disease onset was significantly shorter in PGN patients with than without mesangial VPF/VEGF expression (p<0.01). These findings provide the first evidence that activated MC are a source of VPF/VEGF in human PGN,and indicate that mesamgial VPF/VEGF expression is characteristic of early lesions of PGN.As VPF/VEGF plays a pivotal role in tissue repair, MC-produced VPF/VEGF may play pathophysiological roles, including promoting recovery from glomerular injuries, in early-stage PGN. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Iijima K et al.: "Multiple combined therapy for severe Henoch-Schonlein nephritis in children" Pediatric Nephrology. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 飯島 一誠 他: "活性化メサンギウム細胞による血管透過性亢進因子(VPF/VEGF)の産生とその病態生理学的意義" 日本小児腎臓病学会雑誌. 10(1). 22-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iijima K et al.: "Activation-induced expression of vascular permeability factor by human peripheral T cells:a non-radioisotopic semiquantitative reverse transcription-polymerase chain reaction assay" Journal of Immunological Methods. 196. 199-209 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 飯島 一誠: "小児期IgA腎症の発症進展機序と最新の治療法" 日本小児科学会雑誌. 100(9). 1445-1448 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iijima K.et al.: "Multiple combined therapy for severe Henoch-Schonlein nephritis in children." Pediatric Nephrology. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iijima K.et al.: "Activated mesangial cells produce vascular permeability factor/vascular endothelial growth factor (Japanese)" The Journal of the Japanese Society of Pediatric Nephrology. 10 (1). 22-26 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iijima K.: "Childhood IgA nephropathy : the mechanisms of progression and a new effective therapy (Japanese)" The Journal of the Japan Pediatric Society. 100. 1445-1448 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iijima K et al.: "Activation-induced expression of vascular permeability factor by human peripheral T cells a non-radioisotopic semiquantitative reverse transcription-polymerase chain reaction assay." Journal of Immunological Methods. 196. 199-209 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iijima K et al.: "Multiple combined therapy for severe Henoch-Schonlein nephritis in children" Pediatric Nephrology. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 飯島一誠他: "活性化メサンギウム細胞による血管透過性亢進因子(VPF/VEGF)の産生とその病態生理学的意義" 日本小児腎臓病学会雑誌. 10(1). 22-25 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] IIJIMA K,YOSHIKAWA N NAKAMURA H.: "Activation-inducel expression of vascular permoability factor by human pairplevel T cells : A non-radioisofopic sewiquantitative reverse transeription-polymerase chaim reaction assay" Journal of Immunological Methods. 196. 199-209 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 飯島一誠 苅谷誠子 中村肇 黒田直人 村祥剛 伊東完 吉川徳茂: "活性化メサンギウム細胞による血管透過性亢進因子(VPF/VEGF)の産生とその病態生理学的意義" 日本小児腎臓病学会雑誌. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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