| Project/Area Number |
08671320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Saitama Medical School |
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Principal Investigator |
OGAWA Yunouske Saitama Medical School, Pediatrics, Professor, 医学部, 教授 (90080126)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Koji Saitama Medical School, Pediatrics, Instructor, 医学部, 助手 (30224596)
ITAKURA Yukino Saitama Medical School, Pediatrics, Instructor, 医学部, 助手 (70223071)
ARAKAWA Hiroshi Saitama Medical School, Pediatrics, Instructor, 医学部, 助手 (90271238)
NAKAMURA Toshihiko Saitama Medical School, Pediatrics, Instructor, 医学部, 助手 (30255137)
SHIMIZU Hiroshi Saitama Medical School, Pediatrics, Lecturer, 医学部, 講師 (90260843)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | pulmonary surfactant / functional deficiency / acute respiratory distress syndrome / bronchoalveolar lavage fluid / superoxide anion / superoxide dismutase / newborn infant / スーパーオキシドジムスターゼ / 化学伝達物質 |
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| Research Abstract |
In order to elucidate the pathophysiology of functional deficiency of pulmonary surfactant in the newborn infants, an animal model of acute respiratory distress syndrome was produced in the newborn piglet by the administration of endotoxin (lipopolysaccharides : LPS) via trachea. In this animal model, multiple organ failure was eliminated and solely the lung pathology similar to human ARDS due to septicemia could be achieved. The lung pathology was confirmed by the decreased surface activity, progressive hypoxemia and hypercarbia, decreased lung compliance, increased O_2 productivity, increased elastase activity, and increased lipid peroxides in bronchoalveolar lavage fluid (BAIF). Lung pathology was confirmed also by histological examination on autopsy.
Then, the effect of prophylactically and therapeutically administered recombinant human superoxide dismutase (rh-SOD) on acute lung injury was evaluated. The prophylactic intratracheal administration of rh- SOD for lung injury caused by endotoxin was effective, It was confirmed by the maintenance of normoxemia and normocapnia, and lung compliance, and decreased production of lipid peroxides. Histologic examination also showed that the degrees of lung injury were milder in SOD pretreated animals.
However, after the formation of lung injury, the intratracheal administration of rh-SOD was no more effective on the improvement.
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