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Basic research for cancer-specific immunotherapy using T cell receptor

Research Project

Project/Area Number 08671369
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YAMAGUCHI Oshiyuki  Research Institute for Radiation Biology and Medicine, HIROSHIMA UNIVERSITY Assistant professor, 原爆放射能医学研究所, 講師 (10230377)

Co-Investigator(Kenkyū-buntansha) MIYAHARA Eiji  Hiroshima University Medical Hospital, Medical stuff, 医学部・附属病院, 医員
野間 浩介  広島大学, 医学部・附属病院, 医員
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsCTL / T cell receptor (TCR) / malignant effusion / T細胞受容体 (TCR)
Research Abstract

Locoregional lymphocytes in the ascites caused from colon cancer were isolated and expanded with interleukin (IL)-2 to be potent effector cells against autologous tumor cells. They were CD4 and Fas-L positive, and produced TNF and IFN-gamma. Their cytotoxicity against autologous tumor cells was abrogated with anti-TCR V beta 20 as well as anti-TCR a alpha beta antibody. This TCR V beta 20 gene was cloned and the CDR3 sequence was identified. Molecular diagnosis for locoregional lymphocytes from cancer patients by PCR using the CDR3 sequence as a primer revealed that 3 of 4 patients with positive response to immunotherapy showed positive bands and 3 of 3 patients with negative response did negative ones. These evidences indicates the possibilities of cancer-specific immunotherapy using the TCR V beta 20 and molecular diagnosis for responder patients against cancer-specific immunotherapy.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 山口佳之: "癌に対する免疫遺伝子治療" 癌治療と宿主. 10. 463-470 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口佳之: "TK遺伝子導入による癌治療-IL-2遺伝子共導入による効果増強-" 臨床外科. 53. 1063-1066 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口佳之: "自己リンパ球を用いた癌治療" MINOPHAGEN MEDICAL REVIEW. 43. 321-326 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口佳之: "免疫治療とその後の展開-遺伝子治療-" Practical Oncology. 10. 20-21 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口佳之: "消化器癌治療とサイトカイン" 外科治療. 76. 68-74 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Eiji Miyahara: "Profile of T-cell receptor V β gene usage of cytotoxic T cells induced by intraperitonea administration of a straptococcal preparation,OK-432,in malignant effusions" J.Exp.Therapeutics Oncol.1. 242-250 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi Y,et al: "Immuno-gene therapy of cancer." Gan Chiryo to Shukushu. 10. 463-470 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi Y,et al: "Cancer treatment using TK gene transfer.-Enhancing effect of IL-2 gene-cotransduction-" Rinsho Geka. 53. 1063-1066 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi Y,et al: "Cancer treatment using autologous lymphocytes." Minophagen Med Rev. 43. 321-326 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi Y,et al: "Immunotherapy and current status.-Gene therapy-" Practical Oncol. 10. 20-21 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi Y,et al: "Treatment of gastrointestinal cancer and cytokines" Geka Chiryo. 76. 68-74 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyahara E,Yamaguchi Y,et al: "Profile of T-cell receptor V beta gene usage of cytotoxic T cells induced by intraperitoneal administration of a streptococal preparation, OK-432, in malignant effusions." J Exp Therapeutics Oncol. 1. 242-250 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口佳之: "癌に対する免疫遺伝子治療" 癌治療と宿主. 10. 463-470 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口佳之: "TK遺伝子導入による癌治療-IL-2遺伝子共導入による効果増強-" 臨床外科. 53. 1063-1066 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口佳之: "自己リンパ球を用いた癌治療" MINOPHAGEN MEDICAL REVIEW. 43. 321-326 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口佳之: "免疫療法とその後の展開-遺伝子治療-" Practical Oncology. 10. 20-21 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口佳之: "消化器癌治療とサイトカイン" 外科治療. 76. 68-74 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Eiji Miyahara: "Profile of T-cell receptor V β gene usage・of cytotoxic T cells induced by intraperitoneal administration of a streptococcal preparation, OK-432, in malignant effusions" J.Exp.Therapeutics Oncol.1. 242-250 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口佳之: "B7" Surgery Frontier. 4. 269-271 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口佳之: "免疫療法とその後の展開-遺伝子治療-" Practical Oncology. 10. 20-21 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口佳之: "消化器癌治療とサイトカイン" 外科治療. 76. 68-74 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口佳之: "OK-432免疫療法のresponderの探索と作用機序の解明" Biotherapy. 10. 42-49 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口佳之: "癌性腹水に対するBRM局所投与の臨床効果-臨床試験に向けての課題-" Biotherapy Today. 3. 79-82 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyahara Eiji: "Profile of T-cell receptor Vβ gene us age of cytotoxic T cells induced by in trapleural administration of a streptococcal preparation, OK-432 in malignant effusions" J.Exp.Ther.Oncol.1. 242-250 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口佳之・他: "OK-432免疫療法のresponderの探索と作用機序の解明" Biotherapy. 10. 42-49 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山口佳之・他: "癌性腹水に対するBRM局所投与の臨床効果-臨床試験に向けての課題-" Biotherapy Today. 3. 79-82 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miyahara E., Yamaguch Y.et al: "Profile of T-cell receptor V_β gene usage of cytotoxic T cells induced by intraperitoneal administration of a streptococcal preparation, OK-432, in malignant effusions" J.Exp.Therapeutics Oncol.1. 242-250 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 峠 哲哉 山口佳之: "がん性胸腹水に対するサイトカイン療法・消化器疾患とサイトカイン" へるす出版, 228 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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