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THE EXPRESSION AND ENHANCEMENT OF CO-STIMULATORY MOLECULE (B7-1, B7-2) IN GASTRO-INTESTINAL CANCER.

Research Project

Project/Area Number 08671377
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HARAGUCHI Masaru  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY LECTURE, 生体防御医学研究所, 講師 (40228531)

Co-Investigator(Kenkyū-buntansha) HATAKENAKA Masamitsu  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSISTANT, 生体防御医学研究所, 助手 (40253413)
YAMAGATA Motoyuki  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSITANT, 生体防御医学研究所, 助手 (90294975)
馬場 欽也  九州大学, 生体防御医学研究所, 助手 (30271117)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsGASTROINTESTINAL CANCER / TUMOR IMMUNOLOGY / CO-STIMULATORY MOLECULE / CYTOKINE / 消化器癌 / 接着因子 / RT-PCR法 / B7-1 / B7-2 / Flow cytometry
Research Abstract

The co-stimulatory molecule (B7-1, B7-2) has been shown to play an important role in the induction of T cell mediated immunity against tumors. In the present study, we examined the expression of B7-1 and B7-2 in the human esophageal, gastric and colon cancer cell lines for mRNA level by a reverse transcriptase/polymerase chain reaction analysis (RT-PCR), for their surface expression by a flow-cytometric analysis with monoclonal antibodies. The expression of mRNA for B7-1 or B7-2 was detected in all 18 cell lines tested, except for B7-2 on one cell line. The cells from 13 (72%) or 12 (67%) of these cell lines expressed the surface B7-1 or B7-2 antigen, detected with respective mAb. The surface expression of B7-1 or B7-2 was increased in four to five of the six cell lines tested after a culture with interferon-gamma (IFN-g). In addition, the up-regulation of B7-1 or B7-2 expression by IFN-g was inhibited by interleukin-10 (IL-10). These results indicated that, in the cell lines derived from gastrointestinal carcinoma, both the co-stimulatory molecules B7-1 and B7-2 were detectable in the majority of the cell lines examined at the mRNA and protein levels, and could be regulated with cytokine IFN-g and IL-10.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Haraguchi, M.: "Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma." Int J Oncol. 10. 493-496 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Haraguchi, M.: "Clinical significance of tissue inhibitor of metalloproteinase expression in gastric carcinoma." Br J Cancer. 76. 531-536 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Haraguchi, M.: "Analysis of ornithine decarboxylase mRNA expression in colorectal carcinoma." Dis Colon Rectum. 40. 1095-1100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Haraguchi, M.: "Clinical significance of integrin alpha 6 mRNA expression in gastric carcinoma." Int J Oncol. 11. 959-964 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Haraguchi, M.: "Expression of MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mimori M., Mori M., Ueo H., Koba F., Nakashima H., Baba K., Haraguchi M., Akiyoshi T.: "Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma." Int J Oncol. 10. 493-496 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mimori K., Mori M., Shiraishi T., Baba K., Haraguchi M., Abe R., Ueo H., Akiyoshi T.: "Clinical significance of tissue inhibitor of metalloproteinase expression in gastric carcinoma." Br J Cancer. 76. 531-536 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mimori K., Mori M., Shiraishi T., Fujie T., Baba K., Kusumoto H., Haraguchi M., Ueo H., Akiyoshi T.: "Analysis of ornithine decarboxylase mRNA expression in colorectal carcinoma." Dis Colo Rect. 40. 1095-1100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mimori K.Mori M., Begum N.A., Tanaka S., Haraguchi M., Ueo H., Karimine N., Akiyoshi T., Barnard G.F.: "Clinical significance of integrin alpha 6 mRNA expression in gastric carinoma." Int J Oncol. 11. 959-964 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li J., Yang Y., Fujie T., Tanaka F., Mimori K., Haraguchi M., Ueo H., Mori M., Akiyoshi T.: "Expression of MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Haraguchi,M.: "Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma." Int J Oncol. 10. 493-496 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Haraguchi,M.: "Clinical significance of tissue inhibitor or metalloproteinase expression in gastric carcinoma." Br J Cancer. 76. 531-536 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Haraguchi,M.: "Analysis of ornithine decarboxylase mRNA expression in colorectal carcinoma." Dis Colon Rectum. 40. 1095-1100 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Haraguchi,M.: "Clinical significance of integrin alpha 6 mRNA expression in gastric carcinoma." Int J Oncol. 11. 959-964 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Haraguchi,M.: "Expression of MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Li,J.: "The expression of costimulatory molecules CD80 and CD86 in human carcinoma cell lines : its regulation by interleukin-10." Cancer Immunol.43. 213-219 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Li,J.: "Expression of BAGE,GAGE and MAGE genes in human gstric carcinoma." Clin.Cancer Res.2. 1619-1625 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka,F.: "Efficient induction of anti-tumor cytotoxic T lymphocytes from a healthy donor using HLA-A2-restricted MAGE-3 peptide in vitro." Cancer Immunol.Immunother.(in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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