| Co-Investigator(Kenkyū-buntansha) |
URAGUCHI Takashi Fujita Health Univ.Hospital, the medical staff., 医員 (70288489)
TANIGUCHI Masami Fujita Health Univ, the medical department, Assistant., 医学部, 助手 (40278297)
FUNABIKI Takahiko Fujita Health Univ, the medical department, Professor., 医学部, 教授 (40084537)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
Using a newly established rabbit model of peritoneal carcinomatosis, the mechanism of the effect of thermo-chemotherapy against this pathological status was investigated.
To make the peritoneal carcinomatosis in a rabbit, 1*10^6 Vx2 cells were injected intra-peritoneally. Three weeks afterward, the rabbit was laparotomized under anesthesia and 30 minutes of contineous peritoneal irrigation was conducted using the following agents ; e.g., the group A using normal saline of 38ﾟC,the group B using heated saline of 43ﾟC,the group C using 38ﾟC saline with 20 mg of CDDP and the group D using 43ﾟC saline with 20 mg of CDDP.
Results : 1) Gross assessment of clinical efficacy resulted PDs in all of the A Group (n=6), 11 PDs and 4 NCs out of 15 in the B group, 4 PDs and 6 NCs out of 10 in the C group and 8 NCs and 3 PRs out of 11 in the D group. 2) BrdU L.I.was 12.6(]SY.+-。[)7.6 in the A group, 13.8(]SY.+-。[)4.8 in the B group, 15.2(]SY.+-。[)5.0 in the C group and 20.6(]SY.+-。[)4.0 in the D group. 3) Ki-67 L.I.was 29.3(]SY.+-。[)6.2 in the A group, 32.0(]SY.+-。[)7.6 in the B group, 29.2(]SY.+-。[)3.7 in the C group and 42.8(]SY.+-。[)5.2 in the D group. 4) Expression of the apoptosis was examnined by means of TUNEL methods. A poptotic index was 3.8(]SY.+-。[)2.8 in the A group, 11.0(]SY.+-。[)6.3 in the B group, 18.6(]SY.+-。[)9.8 in the C group and 38.0(]SY.+-。[)29.4 in the D group. 5) The serum concentration level of Pt was higher in the D group whereas the tissue concentration level of Pt was higher in the C group. 6) The serum creatinin, BUN and potassium were higher in both C and D group.
Conclusion : The ratio of the proliferative tumor cells was increased and, at the same time, apoptosis was highly observed in the D group that might imply the affect of thermo-chemotherapy upon tumor cell cycle induced the apoptosis.
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