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Epithelial differentiation and energy production in ulcerative colitis

Research Project

Project/Area Number 08671405
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionTohoku University

Principal Investigator

FUKUSHIMA Kouhei  Tohoku University, Dept.of Medicine, Research Associate, 医学部, 助手 (20271900)

Co-Investigator(Kenkyū-buntansha) FUNAYAMA Yuuzi  Tohoku University, Dept, of Medicine, Lecture, 医学部・附属病院, 講師 (50192315)
NAITOU Hiroo  Tohoku University, Dept, of Medicine, Lecture, 医学部・附属病院, 講師 (90180223)
SASAKI Iwao  Tohoku University, Dept.of Medicine, Associate Professor, 医学部, 助教授 (60125557)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsinflammatory bowel disease / ulcerative colitis / epithelial cell / differentiation / cytokine / alkaline phosphatase / アルカリフォスファターゼ / 上皮細胞 / エネルギー / butyrate
Research Abstract

The purpose of the present study is to investigate potential of LPS and proinflammatory cytokines for energy production in intestinal pithelial cells (IEC). When the cells were stimulated by LPS,IL-6 or TNF-a, ATP amount significantly increased after 6 hours. Chronological enhancement of oxgen consumption, which was completely blocked by antimycin A,was also demonstrated, indicating that LPS and proinflammatory cytokines modulate energy metabolism of IEC.These flexible capacities to generate energy appear to be quite essential for epithelial functions in inflamed mucosa.
To establish markers for epithelial differentiation, the activity of alkaline phosphatase (ALP) was investigated. Butyrate-induced alkaline phosphatase activity is closely associated with differentiation-like phenomena in IEC6 cells, although the type of isoenzyme and cellular localization of the activity are different from those observed in mucosa. When LPS was additionally used with butyrate or retinoic acid, synergistic induction of ALP activities was demonstrated. Other bioactive chemicals such as muramyl peptides (MDP) or N-formylmethionyl-leycyl-phenylalanine (FMLP) failed to exhibit further induction of the activities. The present study clearly demonstrated that the specific combination of bacterial products synergistically modulated ALP activity of intestinal epithelial cells, which is known to be an epithelial differentiation-associated marker.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 福島 浩平: "Lipopoly saccharide exhibits synergishec enhancement of batyrate-induced and retinoic acid-mediated alRaline phosphitaee acfivity on small intestinal call line" Digestion. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 福島 浩平: "Sodium bufyrate-induced liver-type alkaline activity in a small intestinal call lire,IEC6" Digestive Digease and Scieuce. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 福島, 浩平: "上皮細胞を標的とした潰瘍性大腸炎の病態解明と外科的治療体系確立への取り組み" Progress in medicine. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 福島 浩平: "かなえ医学助成金.受賞者研究業績集 第24集 大腸全摘後の残存小腸上皮の適応と促進するための基礎的検討" かなえ医学振興財団, 400(うち23ページ) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kouhei Fukushima: "Lipopolysaccharide and proinflammatory cytokines induced energy production in colonic epithelial cells." (submited for publication).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kouhei Fukushima: "Sodium butyrate-induced liver-type alkakine phosphatase activity in a small intestinal epithelial cell line, IEC6" Dig.Dis.Sci.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kouhei Fukushima: "A trial study to evaluate the pathogenesis and establish surgical manegement for ulcerative colitis, from the view point of epithelial cells." Progress in Medicine. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kouhei Fukushima: "A basic reserch for induction of the adaptation of remmant small intestinal epithelial cells after total colectomy" The 24th Kanae Medical Subsidy The Kanae Medical foundation for the Advancement of Medicine. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 福島浩平: "Lipopoly saccharide exhibits synergistic Inhance ment of butyrate induced-and retinocc acid mediated- alkaline phosphatase activity on small intectinat cell line" Digestion. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 福島浩平: "Sodium butyrate-induced liver-type alkaline phosphatase activity in a small intestinal epithelial cell line,IEC6" Digestive Disease and Science. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 福島浩平: "上皮細胞を標的とした潰瘍性大腸炎の病態解明と外科的治療体系確立への取り組" Progress in medicine. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 福島浩平: "かなえ医学助成金、受賞者研究業績集 第24集 大腸全摘後の残存小腸上皮の適応を促進するための基礎的検討" かなえ医学新興財団, 400(23) (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K. Fukushima et. al.: "Energy Production induced by lipopolysaccharide (LPS) and prainflammatory cytokines in intestinal epithelial cells" Gastroenterology. 110. -A911 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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