Fundamental experiments of bacterial translocation in clinical setting (New development for diagnosis of bacterial translocation)
| Project/Area Number |
08671436
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
HANASAWA Kazuyoshi Shiga Univ.of Med.Sci.1st Dept.of Surg, 医学部, 講師 (80146406)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Hajime Shiga Univ.of Med.Sci.1st Dept.of Surg, 医学部, 助手 (80283563)
ENDO Yoshihiro Shiga Univ.of Med.Sci.1st Dept.of Surg, 医学部, 助手 (40263040)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Endotoxin / Bacterial Translocation / Endotoxin Translocation / mesentric lymph node / Polyethylene glycol / TNF / m-RNA / hemorrhagic shock / Bacterial translocation / Endotoxin / septic shock / Peptidoglycan |
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| Research Abstract |
[Background] Currently, there is no method of accurately diagnosing bacterial translocation (BT) in humans. Bt may be related to changes in intestinal permeability, Changes in small-intestinal permeability caused by polyethylene glycol(PEG)have commonly been reorted in patients with chronic disease, but not in those with acute diseases. PEG does not exist in vivo. Permeability has been confirmed by measuring urinary excretion, In this study, we examined intestinal tract permeability under conditions required for BT development by using PEG.
[Results] Intestinal permeability was enhanced by endotoxin stimulation in a dose-dependent manner. An increase in intestinal permeability was correlated with the incidence of BT, In the hemorrhagic shock model, oxygen administration inhibited BT development, and intestinal permeability also showed a similar tendency. Therefore, BT may develop extremely quickly, associated with enhancement of intestinal permeability of non-absorbable substances. Blood PEG (4000) levels correlated positively with the grade of invasion and the incidence of BT (Journal of the American College of Surgeons). We also reported the 2 clinical cases, which developed septic shock, believed to have been directly caused by bacterial translocation (Surg Today, Jpn 3 Surg)
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Report
(3 results)
Research Products
(7 results)
