| Project/Area Number |
08671471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine (K.P.U.M.) |
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Principal Investigator |
ITOI Hirosumi K.P.U.M., Dept.of Medicine, Assistant, 医学部, 助手 (80203123)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGISHI Hisakazu K.P.U.M., Dept.of Medicine, Associated Professor, 医学部, 助教授 (40128723)
KUBO Hayazo K.P.U.M., Dept.of Medicine, Assistant, 医学部, 助手 (20225189)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | in situ PCR / gastro-enterological carcinomas / image analysis / camcer proliferation / in situ PCR |
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| Research Abstract |
Concerning inherent karyotypic heterogeneities of solid tumor, we evaluated both the exhibiting number of chromosome 17 and nuclear DNA content on an identical nucleus by multi-parametric analysis in order to demonstrate the alteration in number of chromosome 17 among cytofluorometrically distinct subpopulations.
In the present study concerning colorectal carcinoma, we have collected both of these values sequentially on an identical nucleus by using computer controlled auto-scanning stage.
1) We investigated 8 lesions of surgically resected colorectal carcinomas, which were classified as aneuploid in quantitative DNA analysis and also exhibited an increase of 17-aneusomy nuclei. These observations indicate taht the distribution of number of chromosome 17 reflects an endoreduplication of genome content, yet, it does not alter in accordance with the phase of cell cycle.
2) We introduced the thermal cycler for in situ PCR in 1996. the conditions of in situ PCR method were evaluated such as sample preparations primer selection, condition setting and labeling mmethod of signal.
3) It is necessary to evaluate nuclear DNA content simultaneously in order to assess an essential cytogenetic charge. Althoug both nuclear DNA content and number of a particular chromosome should be evaluated simultaneously on an identical nucleus, we usually could not obtain a successful result, because the denaturation process, which is required in FISH procedure, hinders correct evaluation of nuclear DNA content.
4) We still have the problem concerning in situ PCR method applying solid tumor such as colorectal carcinomas.
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