• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of tumor specific killer T-cells and the cloning of the tumor rejection antigen gene

Research Project

Project/Area Number 08671499
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKurume University School of Medicine

Principal Investigator

YAMANA Hideaki  Kurume Univ., Sch.Med., Dep.Surg., Associate Prof., 医学部, 助教授 (30140669)

Co-Investigator(Kenkyū-buntansha) YAMADA Akira  Kurume Univ., Sch.Med., Dep.Immunol., Lecturer, 医学部, 講師 (50158177)
TOH Uhi  Kurume Univ., Sch.Med., Dep.Surg., Assistant, 医学部, 助手 (60268901)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsEsophageal cancer. / Cancer-specific immunity. / Cytotoxic Tlymphocytes. / Tumor-rejection antigens. / Gene cloning. / Antigenic peptides. / Metastatic lymph nodes. / Cancer vaccine.
Research Abstract

We in vestigated the presence of HLA-class I-restricted and tumor-specific CTL in tumor sites of esophageal can cers. HLA-class I-restricted and tumor-specific CTL lines were established from metastatic sites in 5 of 15 patients (Toh et. al., Cell. Immunol. 177 : 137-143,1997). In contrast to these five cases, there was no TIL proliferation in any of four cases of metastatic LN that had undergone prepperative chemotherapy and/or radiation or in six cases of original esophageal tumors. CTL were not generated from lymphocytes of non-metastatic region al LN in any of five patients. These results suggest the existence of HLA-class I-restricted and tumor-specific CTL in tumor sites of esophageal SCC patients who had no preoperative chemotherapy and/or radiation.
We have identified a gene encoding tumor antigens from cDNA library of esophageal squamous cell carcinomas (SCCs) recognizes by HLA-A2601-restricted CTLs (Shichijo et al., J.Exp. Med. 187 : 277-288,1998). This gene showed no similarity to known sequences, and encoded two (125 and 43 kD) proteins. The 125 kD protein with leucine-zipper motif was expressed in the nucleus of the majority of proliferating cells tested including normal and malignant cells. The 43 kD protein was expressed in the cytosol of most of SCCs from various organs and half of lung adenocarcinomas, but was not expressed in other cancers nor in a panel of normal tissues. The three nonapeptides in the region shared by the two proteins were recognized by the KE4 CTLs, and one of the peptides induced in vitro from PBMCs the CTLs restricted to the autologous tumor cells. The 43 kD protein and this nonapeptide (KGSGKMKTE) may be useful for specific immunotherapy of HLA-A2601^+ epithelial cancer patients.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Chandawarkar, R.Y., et al..: "Endosonography for preoperative staging of specific nodal groups associated with esophageal cancer." World J.Surg.20. 700-702 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toh, U., et al.: "A monoclonal antibody KIS-1 recognizing a new membrane antigen on human squamous-cell carcinoma." Int J Cancer. 66. 600-606 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Bhansali, M.S., et al.: "Pattern of recurrence after extended radical esophagectomy with three-field lymph node dissection for squamous cell carcinoma in the thoracic esophagus." World J.Surg.21. 275-281 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, S., et al.: "Effect of methionine-free total parenteral nutrition and insulin-like growth factor I on tumor growth in rats." Am.J.Physiol.273. 10-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toh, U., et al.: "HLA-class-I-restricted and tumor-specific cytotxic T lymphocytes from metastatic lymphocytes from metastatic lympho nodes of esophageal cancers." Cell.Immunol.177. 137-143 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shichijo, S., et al.: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes." J.Exp.Med.187. 277-288 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shichijo S,Nakao M,and Yamana H,et al: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic Tlymphocytes." J.Exp. Med. 187. 277-288 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, S,Yamana H,Shirouze K,et al: "Effect of methionine-free total parenteral nutrition and insulin-like growth factor I on tumor growth in rats." Am.J.Physiol. 273. 10-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toh U,Yamana H,Nakao M,et al: "HLA class I-restricted and tumor specific cytotoxic T lymphocytes from metastatic lymph nodes of esophageal cancers." Cell.Immunol. 177. 137-143 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Bhansali MS,Yamana H,Shirouze K,et al: "Pattern of recurrence after extended radical esophagectomy with three-field lymph node dissection for squamouse cell carcinoma in the thoracic esophagus." World J.Surg. 21. 275-281 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chandawarkar RY,Yamana H,Fujitoh H,et al: "Endosonography for preparative staging of specific nodal groups associated with esophageal cancer." World J.Surg. 20. 700-702 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toh U,Yamana H,Itoh K,et al: "A monoclonal antibody KIS-I recognizing a new membrane antigen on human squamous-cell carcinoma." Int. J.Cancer. 66. 600-606 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chandawarkar,R.Y.,et al.: "Endosonography for preoperative staging of specific nodal groups associated with esophageal cancer." World J.Surg.20. 700-702 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toh,U.,et al.: "A monoclonal antibody KIS-1 recognizing a new membrane antigen on human squamous-cell carcinoma." Int J Cancer. 66. 600-606 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Bhansali,M.S.,et al.: "Pattern of recurrence after extended radical esophagectomy with three-field lymph node dissection for squamous cell carcinoma in the thoracic esophagus." World J.Surg.21. 275-281 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida,S.,et al.: "Effect of methionine-free total parenteral nutrition and insulin-like growth factor I on tumor growth in rats." Am.J.Physiol.273. 10-16 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toh,U.,et al.: "HLA-class-I-restricted and tumor-specific cytotxic T lymphocytes from metastatic lymphocytes from metastatic lympho nodes of esophageal cancers." Cell.Immunol.177. 137-143 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shichijo,S.,et al.: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes." J.Exp.Med.187. 277-288 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toh,Y.,Itoh,K.et al: "Expression of MAGE-1 gene by esophageal carcinomas." Jap.J.Cancer Res.86. 714-717 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakao,M.,Itoh,K.at al: "HLA A2601-restricted cytotoxic T lymphocytes recognizing a peptide antigen expressed on squamous cell carcinoma." Cancer Res.55. 4248-4252 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toh,H.,Itoh,K.,at al.: "A monoclonal antibody KIS-1 recognizing a new membrane antigen on squamous cell carcinoma." Int.J.Cancer,. 66. 600-606 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Itoh,K.,Imai,Y.,: "In:Melanogenesis and malignant melanoma : biochemistry,cell biology,molecular biology,pathology,diagnosis and treatment." (eds.) Hiri,Y.,Hearing,V.J.,Nakajima,J.,Elsevier Science B.V. Tokyo., 312 (1996)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi