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Role of protease on spinal cord ischemia and reperfusion

Research Project

Project/Area Number 08671530
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionYamaguchi University

Principal Investigator

FUJIMURA Yoshihiko  Yamaguchi University School of Medicine Hostpital, Assistant Professor, 医学部・附属病院, 講師 (60199362)

Co-Investigator(Kenkyū-buntansha) HAMANO Kimikazu  Yamaguchi University School of Medicine, Assistant, 医学部, 助手 (60263787)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsThoraco-abdominal Aneurysm / Spinal cord ischemia / Paraplegia / Protease / Calpain-inhibitor
Research Abstract

We investigated the role of protease (Calpain) on spinal cord ischemia and reperfusion. Japanese white rabbits were anesthetized and ventilated mechanically. 4th left thoracotomy was made for clamping the descending thoracic aorta. Just before aortic clamping, calpain inhibitor-1 (10 mu mol/kg) was administered intravenously in calpain group (n=7). Control group rabbits (n=7) received no inhibitor. After 20 minutes' aortic clamping, clamp was removed and chest was closed. Neurological observation was performed for 48 hours by means of Tarlov's score. 6 of 7 rabbits in control group developed complete paraplegia and remaining rabit developed severe paralysis. In contrast, 3 of 7 rabbits in calpain group were recovered without severe paralysis and only 2 rabbits developed complete paraplegia. There was significant difference between two groups (p=0.017). 150-kD fragment of alpha-calspectin was apparantly dense in complete ischemica spine by western blotting. It suggests that calpain play important role on spinal cord ischemia and reperfusion. Histopathology also revealed the spinal cord injury in control rabbit whereas no injury in calpain group.
We conclude that calpain inhibitor-1 may protect spinal cord during ischemia and reperfusion.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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