| Project/Area Number |
08671567
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | University of Tokyo |
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Principal Investigator |
TANIGUCHI Makoto University of Tokyo, Hospetal Department of Neurosurgery, Assistant Professor, 医学部・附属病院, 助手 (50262001)
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| Co-Investigator(Kenkyū-buntansha) |
MISHIMA Kazuhiko University of Tokyo, Hospetal Department of Neurosurgery, Assistant Professor, 医学部・附属病院, 助手 (00282640)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | heparin-binding EGF-like growth factor / brain tumor / glioma / vascular endothelial growth factor / angiogenesis / cell proliferation / beparin-binding EGF / growth factor / brain tcimor / cellproliferation / 悪性グリオーマ / 細胞増殖 / 血管新生 |
|---|
| Research Abstract |
The objective of the present study is to investigate the role of heparin-binding EGF-like growth factor(HB-EGF), which is highly expressed in glioma cells, in progression of malignant glioma, focusing on its effect on the angiogenesis and cellular infiltration.
(A)Involvement of HB-EGF in proliferation of glioma cells
Applying recombinant HB-EGF(rHB-EGF)to glioma cells facilitated the proliferation and DNA synthesis of those cells. Applying specific antibody against HB-EGF to glioma cells inhibited proliferation down to 70% of the control. These results suggested that HB-EGF is strongly involved in proliferation of glioma as an autocrine proliferative factor.
(B)Involvement of HB-EGF in angiogenesis of glioma
Applying rHB-EGF to cultured glioma cells induced expression of vascular endothelial growth factor(VEGF)mRNA,which was evaluated by Northern hybridization method. The effect was initiated at 3 hours after the application of rHB-EGF and was dose-dependent. Secretion of VEGF protein, which was evaluated by Western blotting anslysis, was also increased by application of rHB-EGF.
The study demonstrated that HB-EGF is not only essential for proliferation of glioma cells but also contributes to angiogenesis of glioma via upregulation of VEGF.
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