| Project/Area Number |
08671615
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
YOSHIDA Kazunari Keio University School of Medicine, Department of Neurosurgery, Assistant Professor, 医学部, 講師 (70166940)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIMORI Hisatsugu Keio University School of Medicine, Department of Neurosurgery, Assistant, 医学部, 助手 (30286489)
SHIMAZAKI Kenji Keio University School of Medicine, Department of Neurosurgery, Assistant, 医学部, 助手 (00265836)
KAGAMI Hiroshi Keio University School of Medicine, Department of Neurosurgery, Assistant, 医学部, 助手 (80255471)
SASAKI Hikaru Keio University School of Medicine, Department of Neurosurgery, Assistant, 医学部, 助手 (70245512)
INABA Makoto Keio University School of Medicine, Department of Neurosurgery, Assistant, 医学部, 助手 (00245507)
近藤 新 慶應義塾大学, 医学部, 助手 (20276273)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | cytokine / neuron / astrocyte / neurotrophic factor / cell adhesion molecule |
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| Research Abstract |
We have demonstrated that production and secretion of neurotrophic factors such as nerve growth factor (NGF) from astrocytes was enhanced by cytokines such as fibroblast growth factor(FGF), interleukin-1beta(IL-1beta), tumor necrosis factor-alpha(TNF-alpha), epidermal growth factor(EGF), transforming growth factor-beta1(TGF-beta1), which are likely to be present in the brain following injury.
We have demonstrated new knowledge mentioned below, from 1996- to 1998.
1. IL-1beta, TNF-alpha and EGF induced selective increase of high-molecular-weight bFGF isoforms in cultured rat hippocampal astrocytes.
2. IL-1beta and TNF-alpha co-operatively enhanced a novel trophic activity of astrocytes for pontine cholinergic neurons.
3. TGF-beta1 enhanced expression of NCAM in cultured rat hippocampal astrocytes.
4. Hepatocyte growth factor(HGF) receptor, c-Met was expressed in cultured rat hippocampal astrocytes. Cytokines enhanced expression of c-Met protein in cultured rat hippocampal astrocytes.
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