• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Spinal cord demyelination with experimental continuous compression in rat

Research Project

Project/Area Number 08671621
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKANAZAWA MEDICAL UNIVERSITY

Principal Investigator

IIZUKA Hideaki  Kanazawa Medical University, Department of Neurosurgery, Professor, 医学部, 教授 (50184346)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Kazuya  Kanazawa Medical University, Department of Neurosurgery, Instructor, 医学部, 助手 (00288292)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Keywordscompressive myelopathy / demyelination / axonal degeneration / 実験脊髄損傷
Research Abstract

To analyze the mechanism of chronic compression myelopathy in human spondylosis, ossification of ligaments, and spinal cord tumors, we tried to make a spinal cord continuous compression model in rats using uterine-cervical dilator Dilapan, which has the character of slow expanding, as compression-materials and investigated the lesions. The spine was pressured continuously with compression-material in the sublaminal epidural space at Th9 vertebral level for 1,2,3,7,14,30 and 90 days. The model was investigated morphologically, by histochemistry, immunohistochemistry, electron microscopy as well as conventional histology. The morphological data were analyzed quantiatively by morphometries.
In this model, Dilapan occupied 24.2% of spinal canal in the cross sections. All rats transiently became spastic paraplegia after the placement of compression-materials, but the palsy was recovered in 80% of the rats. Histologically, at the 1st day, hemorrhagic necrosis of the spinal cord occurred, whic … More h was mostly localized in the pressured sites, i.e.posterior column. In the white matter, a spongy change appeared at 2nd day and lasted until 90th day. The spongy change was not localized at the compression site but distributed diffusely in the white matter of spinal cord, although the change was stronger in deeper parts of white matter than in superficial ones. Histochemistry and immunohistochemistry disclosed that the spongy change of white matter was associated with decrease of axon and neurofilaments, but not with demyelination. Examination of toluidine blue staining of thin sections and electron microscopy demonstrated that main change occurred in the lateral column was the degeneration of axon of myelinated fibers, such as edematous swelling, loss of axon causing myelin-tangles, and detachment from myelin sheath. These axonopathy had been continuously seen as long as 90th day. Changes of myelin, such as fragmentation, splitting, and thinning, were also seen in the lateral column but the frequency was low. Morphometry of diameters of myelinated fibers and thickness of myelin did not indicate any significant change of myelin. Furthermore, release of compression decreased the number of degenerated axons. These findings showed that continuous spinal cord compression model using Dilapan is suitable for the analysis of chronic compression myelopathy, indicating that axonopathy but demyelination may be one of the main changes in chronic compression myelopathy. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 飯塚 秀明: "上位頚椎損傷の検討" 日本パラプレジア医学会雑誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki T.: "Roentgenological study of the sagittal diameter of the cervical spinal canal in normal adult japanese." Neurol.Med.Chir.38. 83-89 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 飯塚 秀明: "各種疾患における脊髄神経根の痛みの分析.良性硬膜外病変" Spine Frontier. 4. 65-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岡本 一也: "脊髄持続圧迫による白質病変の実験的検討" 日本パラプレジア医学会雑誌. 10. 68-69 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iizuka.H.: "Germinoma of the optic nerve." Brain Tumor Pathol.13. 95-98 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 飯塚 秀明: "上位頚髄・頚椎障害(最新脳神経外科学)" 朝倉書店, 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 飯塚秀明: "頚椎前方固定術" 医学書院, 8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iizuka H,et al: "Clinical analysis of the upper cervical injury" J Japan Med Soc Paraplegia. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki T,Kadoya S,Iizuka H: "Roentgenological study of the sagittal diameter of the cervical spinal canal in normal adult Japanese." Neuol Med Chir. 38. 83 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okamoto K,Iizuka H,et al: "Spinal cord white matter lesions with experimental continuous compression in rat." J Japan Med Soc Paraplegia. 10. 68 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iizuka H: "Clinical analysis of pain caused by benign spinal cord and roots lesions" Spine Frontier. 4. 65 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iizuka H,et al: "Germinoma of the optic nerve" Brain Tumor Pathol. 13. 95 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iizuka H.: "Germinoma of the optic nerve" Brain Tumor Pathology. 13. 95-98 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岡本 一也: "脊髄持続圧迫による白質病変の実験的検討" 日本パラプレジア医学会雑誌. 10. 68-69 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sasaki T.: "Roentgenological study of the sogittol diameter of the ceruicul spinal canal in normal adult japanese." Neurol.Med.Chir.38. 83-89 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 飯塚秀明: "上位頚推損傷の検討" 日本パラプレジア医学会雑誌. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 飯塚秀明: "各種疾患における脊髄神経状の痛みの分析、良性硬膜外病変" Spine Froutier. 4. 65-72 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 飯塚秀明: "上位頚椎,頚髄障害,(最新脳神経外科学)" 朝倉書店, 11 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 飯塚秀明: "頚椎前方固定術(脊椎・脊髄の手術)" 医学書院, 8 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岡本一也: "脊髄持続圧迫による白質病変の実験的検討" 日本パラプレジア医学会雑誌. 10(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 飯田隆昭: "頚胸椎移行部mobile schwannoma" 日本パラプレジア医学会雑誌. 10(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 飯塚秀明: "上位頚椎・頚髄障害(最新胞神経外科学)" 朝倉書店, 11 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 飯塚秀明: "上位頚椎の臨床" 南江堂(印刷中), (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi