| Project/Area Number |
08671668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
TAKAHASHI Katsurou Nagasaki University, School of Medicine Hospital, Assistant, 医学部・附属病院, 助手 (00244046)
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| Co-Investigator(Kenkyū-buntansha) |
OHTSURU Akira Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (00233198)
MATSUMOTO Tomoko Nagasaki University, School of Medicine Hospital, Assistant, 医学部, 助手 (30239107)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | chondrocytes / Insulin like growth factor / IGF binding protein |
|---|
| Research Abstract |
We have studied the regulation of insulin-like growth factor binding proteins (IGFBPs ) by insulin-like growth factors (IGFs) in primary cultures of rat articular chondrocytes (RAC). Employing Western-ligand blotting (WLB), immunoprecipitation , RAC were found to secrete IGFBP-5 (29 kDa) and IGFBP-4 (24 kDa) as the predominant IGFBPs, as well as IGFBP-2 (32-30 kDa) and IGFBP-3 (43-39 kDa) as the minor species. Treatment of cells with IGF-I and IGF-II resulted in a dose-dependent increase of IGFBP-5. The increase of IGFBP-5 was intermediated by the type 1 IGF receptor. Northern blotting analysis showed that IGF-I and IGF-II increased steady state levels of IGFBP-5 mRNA,suggesting that the IGF-mediated increase in IGFBP-5 is transcriptionally modulated.
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