| Project/Area Number |
08671671
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
IDE Junji KUMAMOTO Univ., School of Medicine, Dep.Orthop.Surg., assistant, 医学部, 助手 (10253725)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGA Makio KUMAMOTO Univ., School of Medicine, Dep.Orthop.Surg., Lecturer, 医学部・附属病院, 講師 (90145318)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | nerve stretching / brachial plexus / retrograde axonal transport / WGA-HRP / interneural microvascular permeability / interneural edema / 末梢神経伸張 |
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| Research Abstract |
The present study was carried out to clarify the effect of stretching the peripheral nerve on retrograde axonal transport, conduction changes, and intraneural microvascular permeability.
The conjugation of horseradish peroxidase with wheat germ agglutinin (WGA-HRP) was used to identify the effect on retrograde axonal transport of stretching the rat sciatic nerve indirectory by 10% and 20% femoral lengthening with a unilateral external fixator. To investigate the relationship between retrograde axonal transport and blood flow in the stretched nerve, nerve blood flow in the sciatic nerve was measured by a hydrogen washout technique. At 11% strain (20% femoral lengthening), the numbers od horseradish peroxidase-labelled motor neuron cells and nerve blood flow had decreased by 43% and 50%, respectivery. Histological examination demonstrated ischaemic changes, but not mechanical damage. However, at 6% strain (10% femoral lengthening) there were no significant abnormalities.
Conduction changes were evaluated by measuring the latency of evoked potentials which were significantly delayd following brachial plexus stretching in rats, but recovered unless the stretching load was strong enough to cause irreversible nerve damage. Interneural microvascular permeability was examined with an Evans Blue Albumin (EBA) injection. At slight traction, EBA leakage was observed only at the trunk of the brachial plexus and not observed at the root and branch. In consistent with this finding, interneural edema was also observed at the trunk of the brachial plexus under relatively lower load while its root and branch were intact.
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