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Etiological study of the ossification of the spinal ligament.-The immunological and endocrinological studies about general ossification-

Research Project

Project/Area Number 08671697
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionDepartment of Orthopaedic Surgery, Toho University School of Medicine

Principal Investigator

OKAJIMA Yukikazu  Department of Orthopaedic Surgery, Toho University School of Medicine Professor, 医学部, 教授 (60104212)

Co-Investigator(Kenkyū-buntansha) KAKIUCHI Terutaka  Department of Orthopaedic Surgery, Toho University School of Medicine Professor, 医学部, 教授 (40126024)
OKADA Yayoi  Department of Orthopaedic Surgery, Toho University School of Medicine Assistant, 医学部, 助手 (60256758)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
KeywordsOPLL / Estrogen / Cultured cell / Cell growth factor / Cytokine / Mononucleocyte / T cell function / CD4 positive T cell
Research Abstract

We investigated the effect of estrogen as a potential causative for ossification of the posterior longitudinalligament (OPLL). The serum estrogen level was significantly higher in the OPLL patients than controls. Cultured cells derived from OPLL patients had receptors with a higher affinity for estradiol than cells from controls. Cultured cells derived from OPLL patients responded to stimulation by estradiol, elevated the tritiated thymidine uptake and increased the BGP products.
In the study of cell growth factors (TGF beta -1, b-FGF), cultured cells derived from OPLL patients stimulated by estradiol, it was found that productive ratio of TGF beta-1 and b-FGF was increased.
We also studied that proliferative response of peripheral mononuclear cell (PMNC). When PMNC was stimulated by mitogens such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (SACI), and monoclonal antibody such as antiCD3 mAb, the prolifetative response of PMNC from OPLL patients was lower than that from healthy controls. IL-2 production of T cells stimulated by PHA or antiCD3 mAb was lower in OPLL patients than healthy controls.
And then PMNC stimulated by PHA or anti CD3 mAb with or without TGF beta-1 or b-FGF.Both TGF beta-1 and b-FGF, in addition of higher concentration, it found that the proliferative response was suppressed.
It was studied that proliferative response of CD4 T cells purified from PMNC to anti-CD3 mAb is lower in OPLL patients than in healthy controls, too. And IL-2 production of CD4 T cells was also lower in OPLL patients than in healthy controls.
In the Study of cell surface markers, CD3, CD4, CD8, CD19, CD25 were detected by flow cytometry. It was not found between OPLL group and healthy controls that difference about subpopulation of lymphocyte.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 和田明人: "脊柱靭帯骨化症の培養靭帯細胞におけるエストロゲン親和性" 日本整形外科学会雑誌. 69. 440-449 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 馬目晃匡: "脊柱靭帯骨化(OPLL)の成因に関する免疫学的検討" 日本脊柱外科学会雑誌. 7・2. 348-355 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 和田明人: "脊柱靭帯骨化の成因としての性ホルモンの作用" 整形・災害外科. 40. 823-830 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 安田ゆりか: "後縦靭帯骨化症における末梢単核球増殖能について" 東日本整形災害外科学会雑誌. 10・4. 436-439 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akihito Wada: "Affinity of estrogen binding in the cultured spinal ligament cells" Journal of Japan Orthopaedic Association. 69. 440-449 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terumasa Manome: "The role of estrogen in ossification of the posterior longitudinal ligament" The Journal of the Japan Spine Research Sosiety. 7(2). 348-355 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akihito Wada: "An experimental study on cultured cells extracted from human spinal ligaments and its affinity for estrogen" Investigation Committee Report on the ossification of the spinal ligaments of the Japanese Ministry of Public Health and Welfare. 103-105 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terumasa Manone: "An immunological study on ossification of posterior longetudinal ligament" Investigation Committee Report on the ossification of the spinal ligaments of the Japanese Ministry of Public Health and Welfare. 106-108 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akihito Wada: "An effect of sex steroid hormones in patients with spinal ligament ossification" Orthopaedic Surgery and Traumatlogy. 40. 823-830 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terumasa Manome: "Cytokine response of mononucleocyte of patient with ossification of the posterior longitudinal ligament" Investigation Committee Report on the ossification of the spinal ligaments of the Japanese Ministry of Public Health and Welfare. 65-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yurika Yasuda: "Proliferation of mononucleocyte in patient with osiification of posterior longitudinal ligament" Journal of the Eastern Japan Association of Orthopaedecs and Traumatology. 10. 436-439 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 和田明人: "背柱靭帯骨化症の培養靭帯細胞におけるエストロゲン親和性" 日本整形外科学会雑誌. 69. 440-449 (1995)

    • Related Report
      1998 Annual Research Report
  • [Publications] 馬目晃匡: "背柱靭帯骨化(OPLL)の成因に関する免疫学的検討" 日本背柱外科学会雑誌. 7・2. 348-355 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 和田明人: "背柱靭帯骨化の成因としての性ホルモンの作用" 整形・災害外科. 40. 823-830 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 安田ゆりか: "後縦靭帯骨化症における末梢単核球増殖能について" 東日本整形災害外科学会雑誌. 10.4. 436-439 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 馬目晃匡: "脊柱靭帯骨化(OPLL)の成因に関する免疫学的検討-骨化発生に関するエストロゲンの役割について-" 日本脊椎外科学会雑誌.

    • Related Report
      1997 Annual Research Report
  • [Publications] 7・2. 348-355 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 馬目晃匡: "脊柱靭帯骨化(OPLL)の成因に関する免疫学的検討-骨化発生に関するエストロゲンの役割について-" 日本脊柱外科学会雑誌. 7・2. 348-355 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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