• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The analysis of the expression of the adhesion molecules against the operative injury

Research Project

Project/Area Number 08671731
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionJichi Medical School (1997)
Kyoto University (1996)

Principal Investigator

TANIGUCHI Yoshihisa  Medicine Assistant Professor, 医学部, 助手 (20243016)

Co-Investigator(Kenkyū-buntansha) MORI Kenjiro  Kyoto Univ.Medicine Professor, 医学研究科, 教授 (20025620)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsAdhesive molecule / gene expression / inflammation / 免疫応答
Research Abstract

The immune response starts when the tissues are injured by operative procedure. Lympyocytes and neutrophils, then, are attracted at the sites by chemotaxic factors such as interleukin 8 and leukotorienes which the injured cells have released, attach the endotherium through adhesion molecules, and chemotaxs to the infammatory sites. It is reported that many adhesion molecules involve the step of the adhesion with endotherium.
We analyzed the expression of adhesion molecules, LFA-1, CD35 and CR3 on the lymphocytes and neutrophils against operative injury by methods of cellular and molecular biology. The lymphocytes and neutrophils were prepared from the patients under operation, stained with the monoclonal antibodies against the adhesion molecules, and analyzed by flowcytometry. At the same time, mRNA were prepared, and the expression of the genes were assessed. The objects were the patients on whom the operations of laparotomy were performed. The expression of LFA-1 on membrane was decreased 1 hour after the start of the operation, and continued to decrease in 6 hours. The expression of LFA-1 mRNA also was decreased. These results suggest that the decrease of LFA-1 expression may be relate to the manipulation to the abdominal cavity and the induction of inflammation of abdomen. However, we did not recognize the significant change of the expression of CD35 and CR3.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Sasada, T.: "Redox control of resistance to cis-diamminedichloroplatinum(II)(CDDP):Protective against CDDP-induced cytotoxicity" J.Clin.Invest.97. 2268-2276 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y.: "A novel promoter sequence is involved in the oxidative stress-induced expression of the human thioredoxin(Trx)gene" Nucleic Acids Res.24. 2746-2752 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masutani, H.: "Transactivation of an inducible anti-oxidative stress protein,human thioredoxin by HTLV-ITax" Imunol-Lett.54. 67-71 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasada, T.: "Redox control of resistance to cis-diamminedichloroplatinum (II) (CDDP) : Protective against CDDP-induced cytotoxicity" J.Clin.Invest.97. 2268-76 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y.: "A novel promoter sequence is involved in the oxidative stress-induced expression of the human thioredoxin (Trx) gene" Nucleic Acids Res.24. 2746-52 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masutani, H.: "Transactivation of an inducible anti-oxidative stress protein, human thioredoxin by HTLV-I Tax" Immunol-Lett.54. 67-71 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi