| Project/Area Number |
08671765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Wakayama Medical College |
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Principal Investigator |
YAMAMOTO Manabu Wakayama Medical College Department of Anesthesia Assistant Professor, 医学部, 助手 (90281279)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Yoshio Wakayama Medical College Department of Anesthesia Professor, 医学部, 教授 (70115913)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Nitric oxide synthase / vascular tissue / in situ hybridization / immunohistochemistry |
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| Research Abstract |
(1)Endotoxic shock was induced by injecting lipopolysaccharide (LPS) intraperitoneally to Wister rats. Aortae from rats treated with LPS for 4 hours showed hyporesponsiveness to phenylephrine. This hyporeactivity was reversed by administration of N^<omega>-Nitro-L-arginine, an inhibitor of nitric oxide synthase.
(2)The study using in situ hybridization showed that treatment with LPS for 4h induced expression of inducible nitric oxide synthase (iNOS)mRNA in the aortic wall. The signals for iNOS mRNA were localized in the non-vascular smooth muscle cells (VSMCs) in the tunica adventitia, and not in VSMCs in the tunica media.
(3)Immunohisotchemistry study using anti rat iNOS sntibody showed that iNOS-like immunorectivity was identified in the tunica adventitia of aortae from rats treated with LPS.
The findings of the present study confirm that LPS administration induces vascular hyporeactivity which is attributable to iNOS induction. The results in in situ hybridization study and immunohisotochemistry study suggest that iNOS induced in the perivascular non-VSMCs is involved in the pathogenesis of endotoxic shock.
The study on the heterogeneity in nitric oxide synthase induction in vascular tissues from different organs is now under way in our laboratory.
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