CHANGES IN NITRIC OXIDE PRODUCTION DURING SHOCK STATE AND ISCHEMIA-REPERFUSION INJURY
| Project/Area Number |
08671773
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | NIPPON MEDICAL SCHOOL |
|---|
Principal Investigator |
SAKAMOTO Atsuhiro NIPPON MEDICAL SCHOOL DEPARTMENT OF ANESTHESIOLOGY ASSOCIATE PROFESSOR, 医学部, 助教授 (30196084)
|
|---|
| Project Period (FY) |
1996 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
|
|---|
| Keywords | NITRIC OXIDE / ELECTRON SPIN RESONANCE / SHOCK / REPERFUION INJURY / CARDIAC CONTRACTILITY / 電子スピン共鳴 / Hb-NO / スピントラップ |
|---|
| Research Abstract |
Using an electron spin resonance spectroscopy, changes in nitrosyl-hemoglobin (NO-Hb) production during experimental septic shock, hemorrhagic shock and splanchnic ischemia-reperfusion injury were measured, and the relationship between cardiac contractility and NO-Hb production during shock was evaluated. The effects of NO synthetase (L-NAME, L-canavanine) inhibitors and NO scavengers (carboxy-PTIO) on survival time were also evaluated. In experimental shock models using rats, the increase in NO-Hb production was well correlated to the decrease in cardiac contractility, and also correlated to the decrease in the response to cathecolamine stimulation during shock. L-NAME and L- canavanine did not affect the mortality ratio, however, carboxy-PTIO (radical scavenger) significantly improve the mortality. These results suggest that 1) NO-Hb detected in the study mainly indicates the production of inducible NO, 2) adequate dose of constitutive NO production was necessary for treatment of shock, 3) NO-Hb may be useful index for evaluating the cardiac contractility during shock.
|
Report
(4 results)
Research Products
(10 results)
