| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
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| Research Abstract |
Using an electron spin resonance spectroscopy, changes in nitrosyl-hemoglobin (NO-Hb) production during experimental septic shock, hemorrhagic shock and splanchnic ischemia-reperfusion injury were measured, and the relationship between cardiac contractility and NO-Hb production during shock was evaluated. The effects of NO synthetase (L-NAME, L-canavanine) inhibitors and NO scavengers (carboxy-PTIO) on survival time were also evaluated. In experimental shock models using rats, the increase in NO-Hb production was well correlated to the decrease in cardiac contractility, and also correlated to the decrease in the response to cathecolamine stimulation during shock. L-NAME and L- canavanine did not affect the mortality ratio, however, carboxy-PTIO (radical scavenger) significantly improve the mortality. These results suggest that 1) NO-Hb detected in the study mainly indicates the production of inducible NO, 2) adequate dose of constitutive NO production was necessary for treatment of shock, 3) NO-Hb may be useful index for evaluating the cardiac contractility during shock.
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