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Study on the plasticity of ion-channel in the bladder afferent and efferent neurons associated with bladder hypesrefl

Research Project

Project/Area Number 08671812
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KAKEHI Yoshiyuki  Kyoto University Graduate School of Medicine, Assistant Professor, 医学研究科, 講師 (20214273)

Co-Investigator(Kenkyū-buntansha) OKUNO Hiroshi  Kyoto university Graduate School of Med.Instructor, 医学研究科, 助手 (90263079)
OKADA Yusaku  Kyoto University Graduate School of Med.Associate Professor, 医学研究科, 助教授 (20127062)
YOSHIDA Osamu  Kyoto University Graduate School of Med.Professor, 医学研究科, 教授 (70025584)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsmicturition reflex / ion-channel / plasticity / neurotransmitter / nitric oxide / bladder efferent pathway / whole-cell patch clamp / nerve growth factor / イオンチャネル / ホールセルパッチクランプ / 膀胱運動路
Research Abstract

(1) Primary afferent neurons in dorsal root ganglia (DRG) and efferent neurons in major pelvic ganglia innervating the urinary bladder were labeled by retrograde axonal transport of a fluorescent dye (Fast Blue) injected into the bladder wall in female adult rats. Bladder afferent and efferent neurons were then dissociated using enzymatic solution including trypsin and identified by a fluorescent microscope.
(2) Electrophysiological studies using whole-cell patch clamp recording techniques were performed to examine the effects of nitric oxide (NO), which is one of neurotransmitters regulating lower urinary tract function on dissociated bladder afferent and efferent neurons. An application of SNAP,an NO donor, inhibited calcium channel currents by 30% in bladder afferent neurons. These inhibitory effects were mimicked by an application of 8-bromo-cyclic-GMP,membrane permeable cyclic-GMP,and the NO-mediated inhibition of calcium channel currents was then antagonized by an preincubation of ODQ,a NO-dependent guanylate cyclase inhibitor. These results indicate that NO has an inhibitory effect on bladder afferent pathway via cyclic-GMP dependent mechanisms (these data have been presented at 27th Annual meeting of Society for Neuroscience, USA)
Effects of nerve growth factor (NGF) on bladder afferent and efferent neurons were investigated using whole-cell patch clamp recording techniques. In contrast to NO,an application of NGF increased calcium channel currents by 30% in bladder afferent neurons. These facilitatory effects of NGF on calcium channel currents were mainly observed in C-fiber bladder afferent neurons which exhibited tetrodotoxin (TTX)-resistant sodium currents. These results indicate that NGF can modulate excitability of bladder afferent neurons, and therefore might contribute to an emergence of bladder hyperreflexia.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(23 results)

All Other

All Publications (23 results)

  • [Publications] Naoki Yoshimura, Eiji Mizuta, Osamu Yoshida, Sadako Kuno: "Therapeutic effects of dopamine D1/D2 receptor agonists on detrusor hyperreflexia in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned porkinsonian cynomolgus monkeys" Journal of Pharmacology and Experimental Therapeutics.(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, Susan L.Erdman, Michael W.Snider, William C.de Groat: "Effects of spinal cord injury on neurofilament immunoreactivity and capsaicin sensitivity in rat dorsal root ganglion neurons inaervating the urinary bladder." Neuroscience. 83. 633-643 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, William C.de Groat: "Modulation of Ca2+ currents by nitric oxide(NO)donors in rat dorsal root ganglion neurons." Soc.Neurosci.Abstr.23. 1185- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, Yasuhiro Nagahama, Tomohiro Ueda, Osamu Yoshida: "Paroxysmal urinary incontinence associatid with mulciple sclerosis." Urologia Internationalis. 59. 197-199 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, William C.de Groat: "Plasticity of Nat channels in rat afferent neurones innervating urinary bladder following spinal cord injury." Journal of Physiology(London). 503. 269-276 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimio Sugaya, James R.Roppolo, Naoki Yoshimura, John P.Gard, William C.de Groat: "The central neural pathways involved in micturition in the neonatal rat as revealed by the injection of pseudcrabies virus in to the urinary bladder." Neuroscience Letters. 223. 197-200 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, Eiji Mizuta, Osamu Yoshida and Sadako Kuno: "Therapeutic effects of dopamine D1/D2 receptor agonists on detrusorhyperreflexia in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned parkinsonian cynomolgus monkeys." Journal of Pharmacology and Experimental Therapeutics. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, Susan L.Erdman, Michael W.Snider and William C.de Groat: "Effects of spinal cord injury on neurofilament immunoreactivity and capsaicin sensitivity in rat dorsal root ganglion neurons innervating the urinary bladder." Neuroscience. 83. 633-643 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura and William C.de Groat: "Modulation of Ca2+currents by nitric oxide (NO) donors in rat dorsal root ganglion neurons." Soc.Neurosci.Abstr.23. 1185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, Yasuhiro Nagahama, Tomohiro Ueda and Osamu Yoshida: "Paroxysmal urinary incontinence associated with multiple sclerosis." Urologia Internationalis. 59. 197-199 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura and William C.de Groat: "Plasticity of Na+channels in rat afferent neurones innervating urinary bladder following spinal cord injury." Journal of Physiology (London). 503. 269-276 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimio Sugaya, James R.Roppolo, Naoki Yoshimura, John P.Card and William C.de Groat: "The central neural pathways involved in micturition in the neonatal rat as revealed by the injection of pseudorabies virus into the urinary bladder." Neuroscience Letters. 223. 197-200 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Yoshimura, Eiji Mizuta, Osamu Yoshida, Satake Kana: "Therapeutic effects of dopamin D1/D2 receptor agonists on detrusor hyperreflexia in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned parkinsonian cynomolgus monkeys" Journal of Pharmacclogy and Experimental Therapeutics. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naoki Yoshimura, Susan L.Erdman, Michael W.Snider, William C.de Groat: "Effects of spinal cord injury on neurofilament immunoreactivity and capsaicin sensitivity in rat dorsal root ganglion neurons" innervating the urinary bladder. Neuroscience 83. 633-643 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naoki Yoshimura, William C.de Groat: "Modulation of Ca2+ currents by nitric oxide(NO)clonors in rat dorsal root ganglion neurons" Soc.Neurosci.Abstr.23. 1185 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naoki Yoshimura, Yasuhiro Nagahama, Tomohiro Ueda, Osamu Yoshida: "Paroxysmal urinary incontinence associatid with multiple sclerosis" Urologia Internationalis. 59. 197-199 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naoki Yoshimura, William C.de Groat: "Plasticity of Na+ channels in rat afferent neurones innervating urinary bladder following spinal cord injury" Journal of Physiology(London). 503. 269-276 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kimio Sagaya, James R.Roppolo, Naoki Yoshimura, John P.Gard, William C.de Groat: "The central neural pathways involved in micturition in the neonatal rat as reveated by the injection of pseudorabies virus in to the urinary bladder" Neuroscience Letters. 223. 197-200 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshimura,N.: "Different types of Na^+ and K^+ currents in dorsal root ganglion neurons innervating the rat urinary bladder." Journal of Physiology(London). 494. 1-16 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshimura,N.: "Characterization of voltage-sensitive Na^+ and K^+ currents recorded from acutely dissociated pelvic ganglion neurons of the adult rat." Journal of Neurophysiology. 76. 2508-21 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshimura,N.: "Neural control of lower urinary tract." International Journal of Urology. 4. 111-125 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshimura,N.: "Plasticity of Na^+ channels in afferent neurons innervating rat urinary bladder following spinal cord injury." Journal of Physiology (London). (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] de Gtoat,W.C.: "In Advance in Neurology" F.J.Seil,New York,Raven Press, 347-364(chapter 33) (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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