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REGULATION OF TRANSCRIPTS ENCODING ION CHANNEL SUBUNITS IN MYOMETRIUM

Research Project

Project/Area Number 08671863
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

TEZUKA Naohiro  YAMAGATA UNIV.SCHOOL OF MEDI.ASSISTANT PROF., 医学部, 講師 (60261690)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsMyometrium / Ion channel / Molecular biology
Research Abstract

Extracellular Ca^<2+> is required for myometrial cells to contract forcefully. Ca^<2+> enters muscle cells mainly through voltage-dependent Ca^<2+> channels (VDCCs) that open in response to action potentials. The synthesis of myometrial VDCCs may change during pregnancy to alter excitation-contraction coupling. We investigated the levels of L-type VDCC alpha1-subunit mRNA in rat and human myometrium to determine whether alterations are associated with term or preterm labor. RNA isolated from myometrial tissues was analyzed by reverse transcription-PCR using specific primers designed according to the published sequence. From pregnant rat myometrium, two distinct PCR products were obtained : one of the expected size at 372 base pairs (bp) and a smaller at 339bp. The two products followed the same trend throughout pregnancy. VDCC mRNA levels increased gradually to 6.9-fold just prior to labor on day 22, but decreased during labor. Similarly ZK98.299 treatment on day 17 caused a statistically significant increase after 8 hours then a decrease during preterm labor at 24 hours. There was no significant increase in animals treated with progesterone from day 19 to day 22. From human myometrium, two PCR products were also obtained. VDCC mRNA levels in the term no labor group were about 4-fold higher than those in the term with labor. We conclude that mRNA levels increase prior to term and preterm labor but decline during periods when VDCCs are likely at their peaks. Progesterone withdrawal or blockade appears to be responsible for regulating the expression of VDCC in preparation for the myometrium to contract.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Nakahara K.et.al: "Incidence of Apoptotic Bodies in Membrana Granulosa of the Patients Participating in an In Vitro Fertilization Program" Fertil.Steril.67・2. 302-308 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tezuka N.et.al.: "Sexual Difference in Early Fetal Crown-Rump Length versus Gestational Agein Pregnancies Arising from in vitro Fertilization" Gynecol.Obstet.Invest.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tezuka N.et.al.: "Development and Sexual Difference in Embryonic Heart Rates in Pregnancies Resulting from in vitro Fertilization" Gynecol.Obstet.Invest.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakahara K.et.al.: "Incidence of Apoptotic Bodies in Membrana Granulosa of the Patients Participating in an In Vitro Fertilization Program" Fertil.Steril.67-2. 302-308 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tezuka N.et.al.: "Sexual Difference in Early Fetal Crown-Rump Length versus Gestational Age in Pregnancies Arising from in vitro Fertilization" Gynecol.Obstet.Invest.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tezuka N.et.al.: "Development and Sexual Difference in Embryonic Heart Rates in Pregnancies Resulting from in vitro Fertilization" Gynecol.Obstet.Invest.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakahara K., et. al.: "Incidence of Apoptotic Bodies in Membrana Granulosa of the Patients Participating in an In Vitro Fertilization Program" Fertil. Steril.67・2. 302-308 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tezuka N., et. al.: "Sexual Difference in Early Fetal Crown-Rump Length versus Gestational Agein Pregnancies Arising from in vitro Fertilization" Gynecol. Obstet. Invest.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tezuka N., et. al.: "Development and Sexual Difference in Embryonic Heart Rates in Pregnancies Resulting from in vitro Fertilization" Gynecol. Obstet. Invest.(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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