| Project/Area Number |
08671868
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
YAMADA Kiyohiko Gunma University School of Medicine, Department of Obstetrics and Gynecology, Assistant Professor, 医学部, 講師 (00210443)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | ovarian follicle / preantral follicle / activin A / Follicle-stimulating Hormone / Transforming Growth Factor / Growth Hormone / follistatin / inhibin / activin / growth hormone / follicle-stimulating hormone / TGF-β / gonadotropin / menarche / differential display / gonadotropin flash |
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| Research Abstract |
There are reports about the maturation and differentiation of the ovaries. However almost all those reports dealt with granulosa cells from estrogen primed animals. So far little was known about the maturation process of ovarian follicles. In this study we established an in vitro culture system of preantral follicles in the mouse.
We found that the preantral follicle from immature mice responded to Activin A, but not to Follicle-stimulating Hormone (FSH), and preantral follicles from mature mice responded to FSH, but not to Activin A (1). Then we found that Growth Hormone (GH) had a direct stimulatory effect on the preantral follicular growth (2), especially to the theca layers of the follicle. Transforming Growth Factor beta 1 (TGF beta 1) had also a stimulatory effect to the preantral follicle growth from mature mice, but not to those from immature mice. We also demonstrated that estrogen priming to the immature mice produced characteristic changes to preantral follicles that did not resemble to any other follicles from non primed control animals (in press). We have found the possibility that Activin A from larger follicles suppresses growth of the surrounding preantral follicles, and removal of this suppression causes de novo follicular growth (3).
We got interesting result about the character changes of preantral follicle from immature to mature type according to the responses to Activin A and FSH, direct effect of follistatin to the preantral follicles, changes of serum concentrations of inhibin A and inhibin B during sex maturation, and so on. They will be published in the future. We inquire further into problems about follicular growth in relation to leptin, neuropeptide Y and adrenal androgens and the onset of sex maturation.
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