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Effect of adrenomedullin on placental and ovarian function

Research Project

Project/Area Number 08671873
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUBOTA Toshiro  Tokyo Medical and Dental Univ.Faculty of Medicine, Assoc.Prof., 医学部, 助教授 (50126223)

Co-Investigator(Kenkyū-buntansha) ASO Takeshi  Tokyo Medical and Dental Univ.Faculty of Medicine, Prof., 医学部, 教授 (60093176)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsAdrenomedullin / Pregnancy / delivery / Maternal plasma / Placenta / Immunohistochemistry / Northern blot / RIA / 分子生物学 / 妊娠・分娩期 / 特異的RIA / 妊娠・分娩・産褥 / 血中濃度
Research Abstract

Adrenomedullin (AM) is a noved vasolelaxtant peptide recently isolated from human phenochromocytoma. AM is found to regulate the cardiovascular system, suggesting that AM has also an important role in the control of blood flow in pregnant women. The present study was undertaken to investigate the possible involvement of AM in human maternal circulation and in placental function during pregnancy. AM concentrations in maternal plasma circulation and in placental function during pregnancy. AM concentrations in maternal plasma were measured by a highly sensitive and specific radioimmunoassay (RIA) for AM using anti-human AM rabbit serum. Immunoreactive (ir)-AM levels in maternal plasma were 3.4 * 0.7 fm ol/mI (mean * SEM) in the first trimester, 3.3 * 1.1 in the second trimester, 7.3 * 2.8 in the third trimester. 4.1 * 1.9 in early puerperium and 3.0 * 0.4 in non-pregnant periods. Ir-AM level in the third trimester was significantly greater than those in other periods. Plasma concentrations of estradio1 (E2), progesterone .(P), human placental lactogen (hPL) and human chorionic gonadotropin (hCG) were also measured by RIA kits. Significant correlations have been demonstrated between the concentrations of ir-AM and those of E2, P.IIPL.So we examined the expression of AM at placenta by using immunohistochemistry and Northern blot analysis in order to demonstrate the correlation between AM and placental function. We found the immunohisrochemical location of AM in the amnion at term and the expression of AM mRNA in human placental tissues using cloned human AM complementary DNA as probe.
The present study is the report demonstrating the immunoreactivity of hAM in maternal plasma during pregnancy, and suggesting the AM possesses the physiologic functions of AM in the maternal circulation and in the development of placenta.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Kobayashi K,Kubota T.et al.: "Immunoreactive adrenomedullin(AM) concentration in maternal plasura during human pregnancy and AM expression in placenta" in submitting.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi K,Kubota T.et al.: "Study on immunoreactive adrenomedullin in maternal plasma during human pregnancy" 10th International Congress of Endocrinology, Abstract. 846 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小林康祐,久保田俊郎 他: "新しい降圧ペプチド・アドレノメデュリンの妊娠期・産褥期における母体血中動態の検討" 日本内分泌学会雑誌. 72・2. 384 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小林康祐,久保田俊郎 他: "血管作動性ホルモン・アドレノメデュリンの妊娠各期・産褥期における母体血中動態と胎盤機能との関連性に関する検討" 日本産科婦人科学会雑誌. 48・Suppl. S-185 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kubora T,Taguchi M,et al.: "Relationship between the release of prolactin and endochelin-1 in human decidualized endometrial cells" European Journal of Endocrinology. 137・2. 200-204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 久保田 俊郎: "卵巣・顆粒膜細胞と子宮内膜からの黄体機能調節機構解明へのアプローチ" 日本産科婦人科学会雑誌. 50・8. 491-499 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 久保田 俊郎: "卵巣 -機能と病態生理-" 医学の世界社(編集企画.吉村泰典), 363 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi K., Kubota T., Aso T., Sato K., Hirata Y., Imai T.and Marumo F.: "Immunoreactive adrenomedullin (AM) concentration in maternal plasma during human pregnancy and AM expression in placenta." (in submitting).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kubota T., Taguchi M., Kobayashi K., Masuda M.and Aso T.: "Relationship between the release of prolactin and endothelin-1 in human decidualized endometrial cells." European Journal of Endocrinology.137. 200-204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masuda M., Kubota T., Kamada S., and Aso T.: "Nitric oxide inhibits stero-idogenesis in cultured porcine granulosa cells." Molecular Human Reproduct-ion. 3(4). 285-292 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masuda M., Kubota T., Kamada S., and Aso T.: "Nitric oxide inhibits stero-idogenesis in cultured porcine granulosa cells." Journal of Reproduction and Development. 43(Suppl). 81-82 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi K., Kubota T.and Aso T.: "A study on the ultrastructural chara-cteristics in the stromal cells of Wharton's jelly in normal pregnancy and EPH gestosis." In 'Recent Advances on the pathophysiology of pregnancy' eds. Nakabayashi M.and Araki T.Organization Gestosis, Tokyo Japan. 181-185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Lin Z., Kubota T., Masuda M.and Aso T.: "Role of nitric oxide synthase in release of endothelin from cultured human endometrial cells." European Journal of Endocrinology. 138(4). 467-471 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi K., Kubota T.and Aso T.: "Study on myofibroblast differentiation in the stromal cells of Wharton's jelly, expression and localization of alpha-smooth muscle actin." Early Human Development. 51. 223-233 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gao Y., Morita I., Maruo N., Kubota T., Murota S.and Aso T.: "Expression of IL-6 receptor and GP130 in mouse bone marrow cells during osteoclast differentiation." Bone. 22(5). 487-493 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi, K., Kubota, T., Hirata, Y., Marumo, F.and Aso, T.: "Study on immun-oreactive adrenomedullin in maternal plasma during human pregnancy." 10th international Congress of Endocrinology, Abstract 846 (3-366), June, San Francisco, USA. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masuda, M., Kubota, T.and Aso, T.: "Effect of nitric oxide (NO) on steroidoge-nesis in porcine granulosa cells in the process of follicular development." 80th Annual Meeting of the Endocrine Society, Abstracts P187 (P1-320), June, New Orleans, USA. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshiki N,Kubota T.Matsumoto Y,Masuda, M.and Aso, T.: "Expression of in-ducible nitric oxide synthase and its role on the release of endothelin-1 from human endometrial cells." 80th Annual Meeting of the Endocrine Socie-ty, Abstracts P 394 (P3-30), June, New Orleans, USA. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Lin,Z.,Kubota,T.et al.: "Role of nitric oxide synthase in release of endothelin from human endonetrial cells" European Journal of Endocrinology. 138・4. 467-471 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kubota,T.,Taguchi,M.et al.: "Relationship between the release of prolactin and endothelin-I in human decidualized endometrial cells" European Journal of Endocrinology. 137・2. 200-204 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi,K.,Kubota,T.et al.: "Study on immunoreactive adrenomedullin in maternal plasma during human pregnancy" 10th International Congress of Endocrinology,Abstrace. 846 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小林康祐 久保田俊郎 他: "新しい降圧ペプチド・アドレノメデュリンの妊娠期・産褥期における母体血中動態の検討" 日本内分泌学会雑誌. 72・2. 384 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小林康祐 久保田俊郎 他: "血管作動性ホルモン・アドレノメデュリンの妊娠各期・産褥期における母体血中動態と胎盤機能との関連性に関する検討" 日本産科婦人科学会雑誌. 48・Suppl. S-185 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久保田 俊郎: "卵巣・顆粒膜細胞と子宮内膜からの黄体機能調節機構解明へのアプローチ" 日本産科婦人科学会雑誌. 50・8. 491-499 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久保田 俊郎: "卵巣 -機能と病態生理-" 医学の世界社 (編集企画 吉村泰典), 363 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Lin Z,Kubota T.et al: "The role of hieric oxide Synthase in release of endoehelin from Cultured haman endomeerial cells" European Journal of Endocrinology. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kobayashi k, Kubota T et al: "Study on immunoredctive adrenomedullin in mdternal plasmd during humdn pregnarcy" loth Interhational Congress of Endocrinology. Abseract. 846 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林康祐, 久保田俊郎他: "新しい降圧ペプチド・アドレノメジュリンの妊娠期・産褥期における母体血中動態の検討" 日本内分泌学会雑誌. 72・2. 384 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubota T, Taguchi M et al: "pelation Ship between the release of Prolactin and Endothelin‐lin human dedidyaliged endometrial cells" European Journal of Endocrinology. 137. 200-204 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林康裕,久保田俊郎他: "血管作動性ホルモン・アドレノメジュリンの妊娠各期・産褥期における母体血中動態と母体機能との関連性に関する検討" 日本産科婦人科学会雑誌. 48・Suppl. S・185 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 久保田俊郎, 増田美香子他: "生殖生理における NO の役割-卵巣機能・受精着床への影響-" 日本産科婦人科栄養・代謝研究会誌. 3・1. 7-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林康祐,久保田俊郎他: "新しい降圧ペプチド・アドレノメジュリンの妊娠期・産褥期における母体血中動態の検討" 日本内分泌学会雑誌. 72・2. 384 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi K,Kubota T et al.: "Study on immunoreactive adrenomedullin in maternal plasma during human pregnancy" 10th international Congress of Endocrinology Abstrace. 846 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小林康祐,久保田俊郎他: "血管作動性ホルモン・アドレノメジュリンの妊娠各期・産褥期における母体血中動態と胎盤機能との関連性に関する検討" 日本産科婦人科学会雑誌. 48.Suppl. S-185 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保田俊郎他: "生殖生理におけるNo9役割-卵巣機能・受精着床への影響-" 第20回日本産科婦人科栄養・代謝研究会抄録集. 15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masuda M,Kubota T et al.: "Nitric oxide inhibits steroidogenesis in porcine granulosa cells" 10th international Congress of Endocrinology Abstrace. 582 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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