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Mechanisms of human papillomavirus E6/E7 expression during cellular differentialtion of cervical cancer

Research Project

Project/Area Number 08671876
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKanazawa University

Principal Investigator

KYO Satoru  University Hospital, Kanazawa University Assistant Professor, 医学部・附属病院, 助手 (50272969)

Co-Investigator(Kenkyū-buntansha) INOUE Masaki  School of Medicine, Professor, 医学部, 教授 (10127186)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHPV / Cervical cancer / Cell differentiation / Transcription factor / 転写調節 / AP1 / YY1 / ヒトパピローマウイルス / 癌遺伝子
Research Abstract

To clarify the molecular mechanisms through which human papillomavirus E6/E7 expression is activated during the process of cellular differentiation in stratified epithelial cells of uterine cervix, we identified core enhancer of upstream regulatory region of HPV3 1 b E6/E7 genes using transient expression assays. DNA foot print analyses and gel shift analyses identified sevral nuclear factors which bind core enhancer and regulate transcription of E6/E7 genes. In particular, Ap1 was found to be the most potent transactivator. In situ hybridization analyses revealed that E6/E7 mRNA was expressed mainly in the undifferentiated basal layers of stratified epithelial cells in uterine cervix, which was co-localized with AP1 expression determined by immunohistochemical analyses. These findings suggest that AP1 is the critical determinant of HPV oncogene expression. In addition, we found that cellular factor YY1 cooperates with API to achieve full activation of HPV oncogene expression. These results may provide insight into molecular targets for gene therapies against cervical cancer induced by HPV infection.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Satoru Kyo,David Masaki Inoue.et al: "Expression of AP1 during cellnlan differentiation determines human papillomarims E6/E7 expression in stratified epithelal all" Journal of General Virology. 78. 401-411 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tan Kanaya, Satoru Kyo Laimins A reimonis: "The 5' region of the human porpilloma Virus type 31 upstream rignlatory region acts as an enbehave which Angments viral early expression" Virology. 237. 159-169 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] "Expression of API during cellular differentiation determines human papillomavirus E6/E7 expression in stratified epithelial cells" Jouenal of General Virology. 78. 401-411 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] "The 5'Region of the Human Papillomavirus Type 31 Upstream Regulatory Region Acts as an Enhancer which Augments Viral Early Expression Thrugh the Action of YY1" Virology. 237. 159-169 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoru Kyo,David Klnmrl,Masaki Inoue,etal: "Expression of AP1 during cellular differentiation determines human papillomarins E6/E7 expression in stratified egiphelinl alls" Journal of General Virology. 78. 401-411 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tan Kanaya,Satoru Kyo Laimoms A Lamms: "The 5'resion of the human papilloma virus type 31 inpstream regnlatory region acts as an enhace which Angments viral emly expression through the action of YY_1" Virology. 237. 159-169 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kyo.S et al: "Transcrptional activity of HPV31b enhancer is regultaed through synergistic interaction of AP1 with two novel factor" Virology. 211. 184-197 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kyo.S et al: "Expression of AP1 dning cellular differentiation determines HPV E6/E7 expression in stratified epithelial cells" J.Gen.Virology. 78. 401-411 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kanaya.T.Kyo.S et al: "The 5'region of HPV31b VRR act as an enhancer which anginents viral expression through the action of YY1" Virology. 237. 159-169 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoru Kyo: "Expression of AP1 during cellular differentlation detenmines HPV E6/E7 expression in stratified epifhelial cells" Journal of General Virology. 78(2). 401-411 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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