| Project/Area Number |
08671892
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
SAWADA Masumi Department of Obstetrics and Gynecology, Osaka University Medical School, Lecturer, 医学部, 講師 (60226074)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Shinzaburo Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseas, 研究所第7部, 主幹
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Gynecologic cancer / Lymph node / Micrometastasis / Genetic diagnosis / RT-PCR / Cytokeratin |
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| Research Abstract |
Usefulness of cytokeratin (CK) 20 mRNA as a target of reverse-transcriptase polymerase chain reaction (RT-PCR) was examined in the detection of gynecologic cancer micrometastases in pelvic lymph nodes (LNs). RT-PCR amplification of CK 20 nRNA was conducted using total RNA samples. RT-PCR products were stained with ethidium bromide and anlyzed by agarose gel electrophoresis. The detection sensitivity of CK 20 RT-PCR was 1/10^5 (cancer cell/ LN cells). One hundred and seventy two LNs were obtained from 37 patients with primary gynecologic cancers (18 cervical, 10 endometrial, and 9 ovarian), and metastases in each LN were investigated by histological examination (hematoxylin and eosin sections) and RT-PCR method. CK20 expression was detected in 47% of the tumor tissues examined by the 1st RT-PCR and in 100% by the 2nd (heminested) RT-PCR.CK 20 mRNA was detected in all of the primary cancer tissues. In all the 5 LNs, which were histologiclly metastases-positive, CK20 mRNA was detected by RT-PCR.Of the 167 histologically negative LNs, 4 LNs were found to express CK 20 mRNA,indicating the presence of micrometastases which could be detected by RT-PCR but not by histological examination. These results demonstrate the usefulness of CK 20 RT-PCR in the detection by gynecologic cancer micrometastases in LNs.
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