| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
To determine ovarian endometriosis specific gene expression, human ovarian tissues obtained on laparoscopy or laparotomy were analyzed by differential display (DD) analysis. Messenger RNA for DD analysis was obtained from ovarian tissues from women obtained informed consent with the diagnosis of ovarian endometriosis and women of the control group, were tested. One 493-bp cDNA band, SCP8-1, was present in ovarian endometriosis, but was absent in ovarian tissues without endometriotic tissue, and that was confirmed by in situ hybridization and Northern hybridization. The differentially expessed cDNA fragment showed highly homology to human serine protease inhibitor (SPI) gene. The SPI mRNA expression was strong and specific in endometriotic epithelial cells and eutopic endometrial glands of endometriosis. In Northern hybridization analysis, SPI mRNA expression of ovarian endometrioma, eutopic endmetrium of endometriosis, and uterine cervix was shown, However, no or little expression was detected in normal endometrium and ovary. SPI codes for a 12-kDa protein, that is found in fluids lining mucosal surfaces, for example, seminal plasma, cervical, nasal, and bronchial mucus, where it regulates both the neutrophil-mediated inflammation that characterizes inflammatory disease, and pathogens themselves via both antiprotease and "defensin-like" activities. According to the immunohistochemistry, SPI staining by Goat anti-human SPI IgG was shown in most of endometriotic epithelial cells and macrophages. SPI is a bacterial lipopolysaccharide(LPS)-induced interferon(IFN)-gamma-suppressible phagocyte product that serves to inhibit LPS responses, therefore, bacterial inflammation may be involved in the pathogenesis of ovarian endometrioma.
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