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Interphase cytogenetic study of apoptosis-related chromosomes and genes

Research Project

Project/Area Number 08671918
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionIwate Medical University

Principal Investigator

MATSUTA Morimasa  IWate Med.Uni.Dept of OB/GYN,associated Prof Pso, 医学部, 講師 (40157326)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsApoptosis / In situ hybridization / Chromosome / Apoptosis / tluorescence in situ hybridization / chromosome / Chromosome / In situ Hybridization
Research Abstract

It was clear that apoptosis plays an important role in cytotoxic mechanisms by anticancer agents. It must be the best way to observe either genetic or morphological changes, when the genetic aberrations during the induction and development of apoptosis are analyzed. We planed the interphase cytogenetic study using Laser Scanning Cytometer (LSC) and Fluorescence In situ Hybridization (FISH). Apoptotic cells are easily recognized morphologically by LSC,and histogram of these cells are analyzable. FISH using whole painting probes revealed that chromosomes 1 and 20 distributed on apoptotic bodies.
FISH observed localization of telomere, which controls life spans of the cells. Telomere was detected on larger apoptoticbodies. On the contrary centromeres did on smaller apoptotic bodies. Hence, morphological changes of apoptosis would start on the centromeres, which locate in the center and are characteristic each of chromosomes, and the phenomenon result into formation of apoptotic bodies.
Cultured cells from human ovarian carcinoma (KFr) and ednometrial carcinoma (Ishikawa) were exposured to CDDP and taxol, and they were harvested for analyzing of apoptotic cells. KFr showed a peak at sub-G1 fraction, and the cells in the fraction were apoptotic cells identified. Further study will follow to identify the apoptosis-related genes.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Morimasa Matsuta et al: "Rewnstructed three-dimentional images of flowsorted nuclei hyloidizal with chomosome L.specific prokes." J Histocham Cytoolam. 44. 1337-1343 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mayumi Matsuta et al: "Immuno his to chomical detection of Ki-67 in epithelial skin fumors im formalin-yixd panaffin xnhe dded tissae sectiono using a new munaclonal antibody(MIB-1)" J Dermatol. 23. 147-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideki Hariu et al: "Cervical uptology by meane of flnorescence in sita hybridization with a set of chromosome spe cific probes" J Obstet Crysecol Res. 22. 163-170 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mayumi Matsuta et al: "Detection of numerical chromosomal akerralione in situ hybridization" J Cutan Pathol. 24. 201-205 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mayumi Matsuta et al: "Immuno his to chemical dete ction of P21/WAF1/C21 I and p53proteins in foralin stixel paraffin em hedded Tosuese ctins of squmscall canamema of the skin" J Derm atol Sci. 14. 233-239 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morimasa Matsuta et al: "Ouarian serous adenocar anome producing Alpha-fctoprotein" J Iwate nied Ass. 49. 589-594 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mo Matsuta, Ma Matsuta, S Hayashi, S Yasumi, K Sasaki, Y kuroda, I Nishiya: "Reconstructed Three-dimensional Images of Flow-sorted Nuclei Hybridized with Chromosome-specific Probes." J Histochm. 44. 1337-1343 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ma Matsuta, S Kimura, G Kosegawa, S Kon, Mo Matsuta.: "Immunohistochemical Detecion of Ki-67 in Epithelial Skin Tumors in Formalin-fixed Paraffin-embedded Tissue Sections Using a New Monoclonal Antibody (MIB-1)" J Dermatol. 23. 147-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Hariu, Mo Matsuta.: "Cervical Cytology by Means of Fluorescence in situ Hybridization with a Set of Chromosome-Specific Probes." J Obstet Gynecol Res. 22. 163-170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ma.Matsuta, Y.Imanura, Mo.Matsuta, K.Sasaki, S.Kon: "Detection of numerical chromosomal aberrations in malignant melanomas using fluorescence in situ hybridization" J Cutan Pathol. 24. 201-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ma.Matsuta, S.Kon, K.Sasaki, Mo.Matsuta: "Immunohistochemical detection of p21WAF1/CIP1 and p53 proteins in formalin-fixed paraffin-embedded tissue sections of squamous cell carcinoma of the skin" J Dermatol Sci. 14. 233-239 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mo.Matsuta, Ma.Matsuta, M.Kagabu, H.Nakata, Y.Utsugisawa, T.Kagabu, I.Nishiya: "Ovarian Serous Adenocarcinoma Producing Alpha-fetoprotein" J.Iwate med Ass.49. 589-594 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松田 壮正: "新しい細胞解析装置" 岩手医科大学圭陵会学内支部会報. 88号. 9-11 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松田 壮正: "α-フェトプロティン産生卵巣漿液性腺癌の一例" 日本(婦人科病理 コルポスコピー学会雑誌. 15巻. 78-82 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mayumi Matsuta et al: "Detection of numerical chromosomal aherretions in madignatl melanoma using thuorescenece in situ hybridization" J Cutan Pathol. 24. 201-205 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mayumi Matsuta et al: "Imonunohisto chemical detection of Pel WAF1/CIP I and p53 proteins in formalin-tixal pantfinemhedded tisanc sections of qusnous cell caveinoca of the skim" J Dermatol Sci. 14. 233-239 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松田 真弓 他: "有棘細胞癌におけるp53蛋白の発現とp53遺伝子変異の解析" 日本皮膚科学会雑誌. 107. 755-760 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morimasa Matsuta et al: "Ovarian serons adenocanainace producing α-feloprotein" J Iwate med Ass. 49. 589-594 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松田 壮正: "Laser Scanning Cytometerによる細胞周期解析 In「フローサイトメトリー,Laser Scanning Cytometer」" 日本サイトメトリー学会, 70 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Matsuta: "Reconstrueted Three-dimentional Images of Flow-sorted Nuslei Hybridiqed with Chromosome-specific prokes" J Histochem Cytochem. 44. 1337-1343 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Matsuta: "Immuno histochemical Detection of Ki-67 in Epithelial Skin Tumors in Formation tixed paraffin emkedded Tisaue Sections using MIB-L" J Dermatol. 23. 147-152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Hariu: "Corvical Cytology by Means of Flusrescence in situ Hybridization with a set of carohosome-specific Prokes" J Obstet Gynecol.Res. 22. 163-170 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松田壮正: "LSM-蛍光インサイツ-ハイブリダイゼーションへの応用" 細胞. 28. 336-339 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松田壮正: "FISH法による染色体分析" 細胞工学. 15. 825-835 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松田壮正: "FISHの細胞診への応用" Medical Technology. 24. 211-215 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松田壮正: "組織細胞化学1996" 日本組織化学会編-学際企画(分担執筆), 208 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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