| Project/Area Number |
08671921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | SAITAMA MEDICAL SCHOOL |
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Principal Investigator |
SEKI Hiroyuki Saitama Medical School, School of Medicine, Associate Professor, 医学部, 助教授 (20179328)
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| Co-Investigator(Kenkyū-buntansha) |
KUROMAKI Ken-ichi Saitama Medical School, School of Medicine, Assistant Professor, 産婦人科, 部長 (20225311)
KINOSHITA Katsuyuki Saitama Medical School, School of Medicine, Professor, 医学部, 教授 (80010354)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Trophoblast / decidua / immortalized cell line / PGE2 / progesterone / DNA / cytokines / TNF-alpha / IL-1β / VEGF / TGF-β / b-FGF / PyNPase活性 / 脱落膜細胞 / 絨毛細胞 / サイトカイン / プロゲステロン / 増殖 / 分化 / 進入 / 不死化細胞 |
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| Research Abstract |
The vector ZipSV4O-6, a recombinant retrovirus derived from ZipNeoSV(X) carrying the SV40 large T gene and the marker gene neomycin acetyl transferase were used to infect primary trophoblast and decidua derived cells, respectivelly. (1)TNF-alpha dose-dependently decreased DNAsynthesis without any effect on progesterone synthe-sis. (2)IL-beta has no effect on both DNA and progesterone synthesis. (3)TCL conditioned medium decreased PGE2 synthesis by DE-1 without affecting DNA content in DE-1. (4)DE-1 conditioned medium increased progesterone synthesis by TCL, whereas that decreased DNA content in TCL.(5)THP-1, which is the cell line derived from monocytic leukemia, attenuated the action of DE-1 on progesterone synthesis and DNA content in TCL.These results indicate that TNF-a regulates the function and proliferation both of decidual and trophoblast cells, that trophoblasts inhibit PGE2 synthesis by decidual cells withou affecting proliferation of decidual cells, and that decidual cells inhibit proliferation of trophoblasts whereas that stimulate progesterone synthesis by trophoblasts. Moreover, it was indicated that THP-1 had effects on the mutual actions between trophoblasts and decidual cells.
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