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Contribution of carbohydrate chains to progression and metastasis of cancer of the endometrium

Research Project

Project/Area Number 08671926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

KUBUSHIRO Kaneyuki  Keio Univ.of Med.Assistant Professor, 医学部, 講師 (50170022)

Co-Investigator(Kenkyū-buntansha) TSUKAZAKI Katsumi  Keio Univ.of Med.Assistant Professor, 医学部, 講師 (40118972)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordscancer of endometrium / carbohydrate chain gene / galactose transferase / gene transfer / infiltration metastasis / 転移 / 糖鎖発現 / β1,4ガラクトース転移酵素 / pCAGGS表現ベクター / ノーザンブロッテイング / ウエスタンブロッテイング / 細胞外基質
Research Abstract

Recently, it has been demonstrated that the carbohydrate chains expressed on cancer cell membranes contribute to tumor metastasis. It has also been reported that changes in the activity of glycosyltransferases involved in the synthesis of carbohydrate chains inhibit experimental metastasis. Accordingly, to determine how type 1 and 2 carbohydrate chains expressed by endometrial cancer are involved in tumor progression and metastasis, we analyzed the mechanism of expression of these carbohydrate chains at the level of galactose transferase, as well as alterations of the carbohydrate chains and tumor cell infiltration after transfer of the gene encoding the galactose transferase synthesizing type 2 carbohydrate chains. We devised a new assay to determine the activities of beta1,3-and beta1,4-galactose transferase (GT), which regulate the synthesis of type 1 and 2 carbohydrate chains. In cancer of the endometrium, beta1,3-GT activity was found to be lower than beta1,4-GT activity. Next, hu … More man beta1,4-GT cDNA was transferred into a cell line derived from endometrial cancer with low expression of beta1,4-GT,yielding a high beta1,4-GT expressing cell line with increased levels of beta1,4-GT mRNA and protein. The expression of a type 1 carbohydrate chain (nLc3Cer) was decreased in the high beta1,4-GT expressing cell line, while the expression of a type 2 carbohydrate chain (Lewis X) was increased. Expression of an adhesion molecule (integrin beta1) was similar in the vector control and high beta1,4-GT expressing cell lines. The high beta1,4-GT expressing cell line showed a high level of cellilar infiltration in vitro. Adhesion of this cell line to extracellular matrix proteins was also increased. These findings suggest that endometrial cancer has lower beta1,6-GT activity than beta1,4-GT activity and that high expression of beta1,4-GT is associated with increased cellular infiltration and adhesion. Accordingly, beta1,4-GT may be involved in the molecular mechanism of infiltration and metastasis of cancer of the endometrium. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kaneyuki Kubushiro et al.: "Expression of glycoconjugates in endometrlal cancer and its association with cell function" International Journal of Gynecologlcal Cancer.7. 91- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "Expression of human β1,4 galactosyltransferase in gynecological cancer cell lines" International Journal of Oncology. 11. 117-122 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "High expression of Uterine Dlphosphate-galactose : Lc3Cer β1,3 galactosyltransferase in human uterine endometrial cancer-derived cells" Japanese Journal of Cancer Research. 887. 669-677 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "Alteration of acidic lipids in human sera during the course of pregnancy" Journal of Chromatography B. 704. 99-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "Expression of glycoconjugates in endometrial cancer and its association with cell function" International Journal of Gynecological Cancer. 7. 91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "Expression of human beta1,4 galactosyltransferase in gynecological cancer cell lines" International Journal of Oncology. 11. 117-122 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "High expression of Uterine Diphosphate-galactose : Lc3Cer beta1,3 galactosyltransferase in human uterine endometrial cancer-derived cells" Japanese Journal of Cancer Research. 88(7). 669-677 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "Alteration of acidic lipids in human sera during the course of pregnancy" Journal of Chromatography B. 704. 99-104 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro et al.: "Expression of glycoconjugates in endometrial cancer and its association with cell function" International Journal of Gynecological Cancer. 7. 91- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kaneyuki Kubushiro et al.: "Expression of human β1,4 galactosyltransferase in gynecological cancer cell lines" International Journal of Oncology. 11. 117-122 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kaneyuki Kubushiro et al.: "High expression of Uterine Diphosphate-galactose : Lc3Cer β1,3galactosyltransferase in human uterine endometrial cancer-derived cell" Japanese Journal of Cancer Research. 88(7). 669-677 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kaneyuki Kubushiro et al.: "Alteration of acidic lipids in human sera during the course of pregnancy" Journal of Chromatography B. 704. 99-104 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 久布白兼行他: "子宮体癌におけるMSN-1認識抗原の組織接着ならびに転移に与える影響" 日本産科婦人科学会会誌. 47. 163-164 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久布白兼行他: "子宮体癌における複合糖質の発現異常とその浸潤・転移に及ぼす影響" 臨床婦人科産科. 49. 225-239 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 福地 剛、久布白兼行 他: "子宮体癌細胞と血管内皮の接着に関与する因子の検討-H型糖鎖を中心として-" 腫瘍マーカー研究会誌. 11. 167-169 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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