| Project/Area Number |
08671950
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Chiba University |
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Principal Investigator |
TERADA Nobuhisa Chiba University, School of Medicine, Department of Otorhinolaryngology, Lecturer, 医学部, 講師 (70197797)
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| Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Masaya Chiba University, School of Medicine, Department of Otorhinolaryngology, Assista, 医学部附属病院, 医員
HIRUMA Kiyoshi Chiba University, School of Medicine, Department of Otorhinolaryngology, Assista, 医学部附属病院, 助手 (30301097)
野本 実 千葉大学, 医学部, 助手 (70237890)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Kimura's Disease / eosinophils / cytokine / chemokine / IL-5 / eotaxin |
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| Research Abstract |
Kimura's disease is a chronic granulomatous disease of unknown etiology. The present study showed that with candida antigen stimulation, the concentrations of IL-5 and IL-5 mRNA in the tumor tissues and lymph node cell suspensions were increased dose- dependently. Stimulation with candida antigen did not produce any obvious change in the concentration of LTB4 or PAF in the culture supernatant. Peripheral blood eosinophils from patients with Kimura's disease showed hypodense and prolonged viability, which suggest that these eosinophils are activated. Almost eosinophils in the tumor tissues and lymph node cells were immunoreactive to anti eotaxin monoclonal antibody.
Eotaxin upregulated the expression of ICAM-1 and VCAM-1 on human microvascular endothelial ceIIsHMMEC,
To understand the potential role of IL-13 in eosinophilic inflammation in Kimura's disease, we examined the effects of IL-13 on the adhesiveness between endothelial cells and eosinophils. IL-13 increased VCAM-1 expression in endothelial cells, the adhesiveness of endothelial cells to eosinophils and the transendothelial migration.
These results strongly indicate that IL-5, IL-13 and eotaxin seemed to be important in eosinophilic inflammation in Kimura's disease.
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