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Expression of fluid regulation peptides in the inner ear.

Research Project

Project/Area Number 08671961
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionShiga Universityof Medical Science

Principal Investigator

KITANO Hiroya  Shiga University of Medical Science, Department of Otolaryngology, Asistant Professor., 医学部, 講師 (20153108)

Co-Investigator(Kenkyū-buntansha) TOOYAMA Ikuo  Shiga University of Medical Science, Department of Molecular Neurobiology Reseac, 分子神経生物学研究センター, 助教授 (20207533)
KIMURA Hiroshi  Shiga University of Medical Science, Department of Molecular Neurobiology Reseac, 分子神経生物学研究センター, 教授 (40079736)
YAZAWA Yoshiro  Shiga University of Medical Science, Department of Otolaryngology, Associate Pro, 医学部, 助教授 (60127040)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Keywordsmolecular biology / inner ear / Meniere's disease / endolymphatic hydrops / inner ear fluid regultion / vasopressin / CNP / VIP / ラット
Research Abstract

The anti-diuretic hormone, vasopressin, has been shown to be important in regulating inner ear fluid. The diuretic hormone, CNP, and its receptor, ANP-B receptor, may also function in the regulation of inner ear fluid. To determine whether vasopressin directly affects the fluid level, we infused this hormone to rat and assay of V2-AVP receptor mRNA by semiquantitative RT-PCR demonstrated a significantly lower level of this transcript in vasopressin-infused animals than in saline-infused animals. The levels of CNP and ANP-B receptors mRNA, however, were the same in both groups of rats. Results suggest that high plasma levels of vasopressin may be a principal causal factor of endolymphatic hydrops in Meniere's disease, perhaps by down-regulating the number of vasopressin receptors.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 水野 博也: "内耳代謝作動ペプチドについて" Otol.Japan. 52. 52-57 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 遠山育夫: "ナトリウム利尿ペプチド受容体" 生命の化学. 49. 401-402 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水野博也: "バソプレッシンによる内耳水代謝の制御" 頭頚部自律神経. 印刷中 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitano. H.: "Presence of mRNA for VIP and its receptor in the rat inner ear" Hear. Res.121. 109-111 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitanishi. T.: "Immurohistochemical detection of VIP and VIP receptor in the rat inner ear" Acta Otolaryngol. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitano, H.: "Regulation of inner ear fluid in the rat by vasopressin" Neuro Report. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzaki M,Kitano H,Kitanishi T,Yazawa Y,Kitajima K,Takeda T,Kimura H,Tooyama I.: "Detection of C-type natriuretic peptide (CNP) and atrial natriuretic peptide (ANP-B) receptor mRNAs in rat inner ear." NeuroReport. 18. 439-443 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitano H,Takeda T,Suzuki M,Kitanishi T,Yazawa Y,Kitajima K,Kimura H,Tooyama I.: "Vasopressin and oxytocin receptor mRNA are expressed in the rat inner ear." NeuroReport. 18. 2289-2292 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki M, Kitano H,Kitanishi T,Yazawa T,Kitajima K,Takeda T,Kimura H,Tooyama I.: "RT-PCR analysis of mRNA expression of natriuretic peptide family and their receptors in rat inner ear." Brain Res Mol Brain Res. 55. 165-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitano H,Takeda T,Suzuki M,Kitanishi T,Yazawa Y,Kitajima K,Kimura H,Tooyama I.: "Presence of mRNA for vasoactive intesinal polypeptide (VIP) and its receptor in the rat inner ear." Hear Res.121 (1-2). 109-111 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitanishi T,Suzuki M,_ Kitano H,Yazawa Y,Kitajima K.: "Immunohistochemical detection of vasoactive intestinal polypeptide (VIP) and the VIP receptor in the rat inner ear." Acta Otolaryngol (supple). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitano H,Suzuki M,Kitanishi T,Yazawa Y,Kitajima K,Takeda T,Kimura H,Tooyama I.: "Regulation of inner ear fluid in the rat by vasopressin." NeuroReport. (in Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 北野 博也: "内耳水代謝作動ペプチドについて" Otol Japan. 52. 52-57 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 遠山 育夫: "ナトリウム利尿ペプチド受容体" 生命の化学. 49. 401-402 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 北野 博也: "バソプレッシンによる内耳水代謝の制御" 頭頚部自律神経. 印刷中 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitano H.: "Presence of mRNA for VIP and its reaptor in the rat mner ear" Hear.Res.121. 109-111 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitaniobi T: "Immunobis to chemical detection of VIP and VIP receptor in the rat mner ear" Acta OTolanyogol. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitano H: "Requlation of mner ear fluid in the rat by vosopressin" Neuro Report. (in press). (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroya KITANO: "Vasopressin and oxytocin receptor mRNA are expresed in the rat iuner ear." Neuro Report. 8. 2289-2292 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mikio SUZUKI: "Detection of C-type natriuretic peptide (CNP) and atrial natriuretic peptide (ANP-B) receptor mRNAs in rat iuner ear." Neuro Report. 8. 439-443 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mikio Suzuki et al: "Detection of c-type natriuretic peptide (CNP) and ANP-B receptor mRNA in rat inner ear" Neuroreport. 8-2. 439-443 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北野 博也、他: "下垂体後葉ホルモンにする内耳水代謝について" Audiology Japan. 39-5. 473-474 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北野 博也、他: "Vasopressinと内リンパ水腫の関係について" 日本耳鼻咽喉科学会会報. 98-10. 110-110 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北野 博也、他: "内耳水代謝作動ペプチドの分子生物学的研究" 日本耳鼻咽喉科学会会報. 99-10. 1606-1607 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroya Kitano,et al: "Expression of water regulation peptides and their receptersin the rat inner ear" Molecular Biology of Hearing and Deatmess. Proci. 92-92 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北野博也、他: "内耳における水代謝作動ペプチドの遺伝子発現" Equilibrium Research. 55-2. 188-189 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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