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Molecular biology of the degenerative and regenerative mechanisms in the inner ear sensory cells

Research Project

Project/Area Number 08671966
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TAKUMIDA Masaya  Hiroshima University Medical Hospital, Assistant professor, 医学部・附属病院, 講師 (00179590)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsinner ear / vestibular organ / cytoskeleton / nitric oxide / otoconia / pathology / molecular biology / カルシウム / モルモット / X線マイクロアナライザー
Research Abstract

The aim of this study is to investigate the degenerative and regenerative mechanisms of inner ear sensory cell in to develop the new treatments for the inner ear diseases.
The effect of ototoxic drugs on the cytoskeletal organization of vestibular sensory cells was investgated. The cytoskeletons were noted to have degenerated following ototoxic drug intoxication. Such degeneration was not linked with primary mitochondrial damage, but closely releated to subsequent degeneration of membrane-bound organelles. These findings suggest that cytoskeletons work closely together to maintain the structural integrity of the membrane-bound organelles, which can be altered by ototoxic drugs before the degeneration of membrane-bound organells.
In the inner ear, constitutive NOS has been identified. NO mediates neurotransmission and play an important role in regulating vascular tone, and in maintaining endolymph and ion homeostasis. In contrast, enhanced NO production by inducible NOS and subsequent for … More mation of peroxynitrite is likely to be an important factor responsible for pathological insult of the vestibuli and removal of NO and/or O_2 to prevent formation of peroxynitrite or removal of peroxynitrite itsel may be beneficial treatment for vestibular diseases.
The otoconial dynamics have been investigated using scanning electron microscopy, x-ray microanalysis and several kinds of Ca tracers. Dynamic exchange and/or uptake of Ca ions may occur even in adult animals. The giant otoconia are suggested to be formed mainly by dissolution of normal otoconia due to the loss of environmental calcium, followed by recrystallization as giant crystals. These phenomena seemed to be closely related to the otoconial dynamics which may regulate calcium ion homeostasis of endolymph.
These results were presented at meetings (Sendai Symposium 96 and 97,5th and 6th meetings of Otological Society of Japan, 55th and 56th meetings of Japan Society for equilibrium Research) and were presented by 21 papers. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (61 results)

All Other

All Publications (61 results)

  • [Publications] 工田 昌也、Anniko M.: "ゲンタマイシンによる前庭感覚細胞の細胞骨格構造の変化." Equilibrium Res. 55. 442-449 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M, Anniko M.: "The effect of gentamicin on cytoskeletons in the vestibular sensory cells : A high-resolution scanning electron microscopic investigation" Acta Otolaryngol (Stockh). 116. 817-823 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M, Anniko M., Yajin K.: "The effect of gentamicin on cytoskeletons in the vestibular sensory cells" Proc Sendai Ear Symposium. 6. 19-22 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 谷川 徹、宮脇 浩紀、工田 昌也、他: "前庭単離有毛細胞の運動性について-アセチルコリンの影響-" Equilibrium Res. 55. 20-25 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang DM, Takumida M., Harada Y.: "Uptake of tetracycline in otoconia of the guinea pig" Acta Otolaryngol (Stockh). 116. 732-736 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang DM, Takumida M., Harada Y.: "Incorporation of tetracycline into otoconia of the guinea-pig following streptomycin intoxication" Acta Otolaryngol (Stockh). 116. 812-816 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang DM, Takumida M., Yajin K., Harada Y.: "Incorporation of calcium into otoconia of the adult guinea pig." Proc Sendai Ear Symposium. 6. 53-57 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM, Yajin K.: "In vitro calcium ion turover in otoconia of the guinea pig" Auris Nasus Larynx. 24. 119-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM, Yajin K., Harada Y.: "Formation and fate of giant otoconia of the guinea pig following streptomycin intoxication" Acta Otolaryngol (Stockh). 117. 538-544 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM, Yajin K., Harada Y.: "Polychromatic labeling of otoconia for the investigation of calcium turnover" ORL. 59. 4-9 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM, Yajin K., Harada Y.: "Effect of streptomycin on the otoconial layer of the guinea pig." ORL. 59. 263-268 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM: "Electron probe x-ray microanalysis of otoconia in the guinea pig inner ear" ORL. 59. 187-192 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM: "Electron probe x-ray microanalysis of otoconia in guinea pig inner ear : A comparison between young and old animals" Acta Otolaryngol (Stockh). 117. 529-537 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM: "NADPH-diaphorase activity in the vestibular end organs of the guinea pig" Otol Jpn. 7. 181-185 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM: "NADPH-diaphorase activity in the endolymphatic sac of the guinea pig" Otol Jpn. 7. 186-190 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Zhang DM, Anniko M.: "Localization of nitric oxide synthase isoforms (I, II and III) in the emdolymphatic sac of the guinea pig" ORL. 59. 311-316 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Anniko M.: "Localization of nitric oxide synthase isoforms (NOS I, II and III) in the vestibular end organs of the guinea pig" ORL. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Anniko M.: "Localization of nitric oxide synthase isoforms (NOS I, II and III) in the vestibular end organs of the guinea pig" Proceeding Sendai Symposium. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M., Anniko M.: "Lipopolysaccharide-induced expression of a nitric oxide syntase II in guinea pig vestibular end organs" Eur Archives Otorhinolaryngol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arishige S., Takumida M., Yajin K.: "Calsium mobilization in isolated epithelial cells of the endolymphatic sac" ORL. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Anniko M., Amold W., Takumida M.: "Human temporal bone sections in immunomorphological research : current status. In Iurato S, Veldman JE. Progress in Human Auditory and Vestibular Histopathology" Kugler Publications bv, Amsterdam, 129-142 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Anniko M: "The effect of gentamicin on cytoskeletons in the vestibular sensory cells" Equilibrium Res. 55. 442-449 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Anniko M: "The effect of gentamicin on cytoskeletons in the vestibular sensory cells : A high-resolution scaanning electron microscopic investigation." Acta Otolaryngol (Stockh). 116. 817-823 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Anniko M,Yajin K: "The effect of gentamicin on cytoskeletons in the vestibular sensory cells." Proc Sendai Ear Symposium. 6. 19-22 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanigawa T,Miyawaki H,Takumida M,et al.: "Motility of isolated vestibular hair cells" Equilibrium Res. 55. 20-25 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang DM,Takumida M,Harada Y: "Uptake of tetracycline in otoconia of the guinea pig." Acta Otolryngol (Stockh). 116. 732-736 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang DM,Takumida M,Harada Y: "Incorporation of tetracycline into otoconia of the guinea-pig following streptomycin intoxication." Acta otolaryngol (Stockh). 116. 812-816 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang DM,Takumida M,Yajin Harada Y: "Incorporation of calcium into otoconia of the adult guinea pig." Proc Sendai Ear Symposium. 6. 53-57 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM,Yajin K: "In vitro calcium ion turnover in otoconia of the guinea pig." Auris Nasus Larynx. 24. 119-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM,Yajin K,Harada Y: "Formation and fate of giant otoconia of the guinea pig following steptomycin intoxication." Acta Otolaryngol (Stockh). 117. 538-544 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM,Yajin K,Harada Y: "Polychromatic labeling of otoconia for the investigation of calcium turnover." ORL. 59. 4-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM,Yajin K,Harada: "ORL" Effect of streptomycin on the otoconial layr of the guinea pig.59. 263-268 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM: "Electron probe x-ray microanalysis of otoconia in the guinea pig inner ear." ORL. 59. 187-192 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM: "Electron probe x-ray microanalysis of otoconia in guinea pig inner ear : A comparison between young and old animals." Acta Otolaryngol (Stockh). 117. 529-537 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM: "NADPH-diaphorase activity in the vestibular end organs of the guinea pig." Otol Jpn. 7. 181-185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM: "NADPH-diaphorase activity in the endolymphatic sac of the guinea pig." Otol Jpn. 7. 186-190 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Zhang DM,Anniko M: "Localization of nitric oxde synthase isoforms (I,II and III) in the endolymphatic sac of the guinea pig." ORL. 53. 311-316 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Anniko M: "Localization of nitric oxide synthase isoforms (NOS I,II and III) in the vestibular end organs of the guinea pig." ORL. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Anniko M: "Localization of nitric oxide synthase isoforms (NOS I,II and III) in the vestibular end organs of the guinea pig." Proceeding of Sendai Symposium. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arishige S,Takumida M,Yajin K: "Calcium mobilization in isolated epithelial cells of the endolymphatic sac." ORL. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M,Anniko M: "Lipopolysaccharide-induced expression of a nitric oxide synthase II in guinea pig vestibular end organs" Eur Archives Otorhinolaryngol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Anniko M,Arnold W,Takumida M: Human temporal bone sections in immunomorphological research : current status. In Iurto S,Veldman JE.Progress in Human Auditory and Vestibular Histopathology. Kugler Publications bv, Amsterdam, 129-142 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumida M, Zhang DM, Yajin K:"In vitro calcium ion turover in otoconia of the guinea pig." Auris Nasus Larynx. 24. 119-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM, Yajin K, Harada Y:"Formation and fate of giant otoconia of the guinea pig following streptomycin intoxication." Acta Otolaryngol (Stockh). 117. 538-544 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM, Yajin K, Harada Y:"Polychromatic labeling of otoconia for the investigation of calcium turnover." ORL. 59・1. 4-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM, Yajin K, Harada Y:"Effect of streptomycin on the otoconial layer of the guinea pig." ORL. 59・5. 263-268 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM:"Electron probe x-ray microanalysis of otoconia in the guinea pig inner ear." ORL. 59・4. 187-192 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM:"Electron probe x-ray microanalysis of otoconia in guinea pig inner ear : A comparison between young and old animals." Acta Otolaryngol (Stockh). 117. 529-537 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM:"NADPH-diaphorase activity in the vestibular end organs of the guinea pig." Otol Jpn. 7・3. 181-185 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM:"NADPH-diaphorase activity in the endolymphatic sac of the guinea pig." Otol Jpn. 7・3. 186-190 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Zhang DM, Anniko M:"Localization of nitric oxide synthase isoforms (I, II and III) in the endolymphatic sac of the guinea pig." ORL. 59・6. 311-316 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Anniko M:"Localization of nitric oxide synthase isoforms (NOS I, II and III) in the vestibular end organs of the guinea pig." ORL. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Anniko M:"Localization of nitric oxide synthase isoforms (NOS I, II and III) in the vestibular end organs of the guinea pig." Proceeding of Sendal Symposium. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Anniko M:"Lipopolysaccharide-induced expression of a nitric oxide syntase II in guinea pig vestibular end organs." (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Arishige S, Takumida M, Yajin K: "Calcium mobilization in isolated epithelial cells of the endolymphatic sac." ORL. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumida M, Anniko M: "The effect of gentamicin cytoskeletons in the vestibular sensory cells" Acta Otolaryngol (Stockh). 116. 817-823 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 工田昌矢、Anniko M: "ゲンタマイシンによる前庭感覚細胞の細胞骨格構造の変化" Equilibrium Res. 55. 442-449 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takumida M, Zhang DM:"Electron probe x-ray microanalysis of otoconia of the guinea pig inner ear" ORL. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Takumida M, et al: "Formation and fate of giant otoconia of the guinea pig following streptomycin intoxication" Acta Otolaryngol (Stockh). (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Takumida M, et al: "Polychromatic labeling of otoconia for investigation of calcium turnover" ORL. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Takumida M, et al: "Effect of streptomycin to the otoconial layer" ORL. (印刷中).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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