| Project/Area Number |
08671998
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
KANOH Naoyuki Hyogo College of Medicine, Otolatyngology, Assistant Pro., 医学部, 助手 (20152829)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Meniere's Disease / Endolymphatic hydrops / Cytochemistry / Na-K ATPase / K-NPPase / Stria vascularis |
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| Research Abstract |
In order to clarify the pathogenesis of Meniere's disease, the relationship between strial Na-K ATPase activity and catecholamines was investigated using a cerium-based cytochemistry. Strial Na-K ATPase activity was affected by exogenous catecholamines and was bidirectionally regulated by catecholamines : dopamine decreased its activity, but other catecholamines and serotonin increased the enzyme activity. Thus, catecholamines are found to be essential for the maintenance of the strial Na-K ATPase activity.
Transport Na-K ATPase activity of the facial nerve was also investigated using the same method mentioned above. Then, two Transport Na-K ATPase reactivities were determined ; reserpine-sensitive and reserpine-resistant. These findings indicated that reserpine-resistant part was independent upon the catecholamines.
After investigating these experiments, the etiology of the endolymphatic hydrops was histologically examined by a light microscope.
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